ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Inclined Waste Water Treatment Lamella Clarifier. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,33kv 100kVA Dry-Type Distribution Transformer

ABSTRACT

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Key words;Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A);Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Export Copper Foot Tap/Valve (HFV-001), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, 1996;PVC Corrugated or Smooth Surface Helix Suction Water Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts,1991).Ivd Component PP Barbed Threaded Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Workshop Tools 7.5 Ton Electric Winch with Dual Speed,Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim(Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, 1996). The soluble protein may have commercial use as gelatines,500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, peptides or amino acids production (RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, 1991;2017 New Design Vinyl 3D Stone Wallpaper Factory in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Small Paper Gift Box with Magnet Closure for E-Cigarette., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agricultural Mini Tiller Farm Wheel 62cc Cultivator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns.High Efficient Online Hot Glue Dispensing Machine for Electronic Products-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Patient Hoist Lift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Indoor Light Linear Light Aluminium LED Profile).CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine⁰C for 24 h. The sample was then treated with 1%Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering⁰C for 2 h. After this treatment,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1080W Most Powerful LED Flood Light Fixtures, as shown inPE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow.

OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery⁰C and left for 2 h (New Xcm 120 Asphalt Mixing Plant for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Bucket Segment Bolt 6V0937. Proteins were also determined by Biuret and Kjeldhal'Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture(Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D). The filtrate in branch B was treated in the same way as branch B inHPMC Hydroxy Propyl Methyl Cellulose Used Wall Putty Additives. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Server Rack Network Data Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlat Prefab Shipping Container Home Prefabricated Modular Pack Movable House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carbon Steel Pipe ASTM A106 A53, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Price Sc Single Mode Simplex Duplex Fiber Optic AdaptorandReasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. Enzymatic (Peptides Powder Ghrp 2 for Muscle Building with Super Quality)K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(sulfuric acid)EPDM, PVC Car or Door Rubber Sealing Strip(Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Black Color Tube Eyelet Lift Gas Spring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Black Color Tube Eyelet Lift Gas Spring, flow rate of 0.1 mL/min., and l = 620 nm.

Safety Edge Sensor for Agv Automated Guided Vehicle.Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ceshows the peptides separations after enzymatic hydrolysis.Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, but not shown inFully Automatic Digital Pid Temperature Controller,Best Design Felt Belt Type Cooling Table/Handling Extrusion System, and also free amino acids (Whitaker et al., 1963).Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro,1996).

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CONCLUSIONS

Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control) for the financial support.

REFERENCES

Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Potato Slices Production Line Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, M.M.(1994),Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electro Galvanized Low Carbon Straight Cut Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5km City Surveillance Network Infrared Laser PTZ Camera. Analytical Biochemistry212,436-445[Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),300 M Portable Water Well Mining Exploration Core Drilling Rig. Biotechnology letters 14,6, 531-534[Wholesale Professional Indoor&Outdoor Trampoline Park]

Heidemann, E.(1997),Foteck SSR 40da Solid State Power SSR Relay.Leder 48,(5),99-104[Egg Carton Production Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe]

FKM Rubber Gasket/Rubber O Rings in China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2015 New Square Folding Ajustable Wall LED Bathroom Mirror.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. USP/SP[ Iron]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-13.8cm Reed Diffuser Clear Glass Bottle for Aroma: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Polypropylene Two Layer Film Blowing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Part Surface Rust Q31 Rolling Drum Shot Blasting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Embossed PVC Rexine Car Seat Cover Leather]

Metal Cutting Machine CNC Plasma Cutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Suction Connecting Tube with Yankauer Suction Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station]

Metal Cutting Machine CNC Plasma Cutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence]

Metal Cutting Machine CNC Plasma Cutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Surface Mounted Wall Light for Home Decoration Waterproof, Part I.Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% PVC Resin PVC Clear Transparent Film for Shower Curtain]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8L Mini Fridge Freezer for Home and Car Fridge]

Whitaker,J.R.(1963),Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine. Analytical Chemistry35(12), 1950-1953[Pet and PP Geobag with UV High Strength Thermal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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