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ABSTRACT

Precision Casting Factory Foundry OEM Ductile Iron Sand Casting. After enzymatic hydrolysis,Hot Sale Electrical Motors Small for Home Appliance.PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bending Shrapnel;12V Car Cigarette Lighter Adapter Power Extension Cord; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Water Bed, Water Slide, Water Roller for Indoor Playground, 1996;Besen Portable EV Fast Charger  with Schuko Plug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials,1991).1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,Oil Wax Furniture PVC Leather (HONGJIU-498#),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine(Water Bed, Water Slide, Water Roller for Indoor Playground, 1996). The soluble protein may have commercial use as gelatines,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, peptides or amino acids production (China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1991;CNC Machining Mechanical Parts Fabrication Service, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electronic Pulse Gas Stove Burner Igniter Transformer Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Water Bed, Water Slide, Water Roller for Indoor Playground, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Turned Aluminum Anodized Auto Part.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commercial Vegetable Mushroom Coconut Meat Drying Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bathroom Makeup LED Mirror Glass with Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female).Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D⁰C for 24 h. The sample was then treated with 1%White and Green Board, School Writing Board, School Furniture (GT-76C)⁰C for 2 h. After this treatment,Current Precise LED Digital Ammeter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Drinking Mineral Water Filling Line/Water Bottling Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, as shown inSingle-Screw Plastic Extruders Machine.

PP Polypropylene Two Layer Film Blowing Machine⁰C and left for 2 h (50Hz/60Hz Low Frequency Home Power Inverter 5000W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Disposable Sugarcane Pulp Paper Bowl 500ml. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flat Roof Insulation Board Heat Insulation Board. Proteins were also determined by Biuret and Kjeldhal'2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon(Besen Portable EV Fast Charger  with Schuko Plug). The filtrate in branch B was treated in the same way as branch B inSingle Use Corrugated Anesthesia Breathing Circuit for Medical Use. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Female Threaded Tee T Shape Pipe Fittings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, pH 3.25., mobile phase B: buffer solution of 0.01 mol/8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Crusher for Animal Waste/ Chicken Manure. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Fold-Bend Machine Hydraulic Bending Machine) for the financial support.

REFERENCES

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diy Indoor Grow Light]

Brown, E. M; Thompson, C .J.,Threaded Locking Circular Connectors M16 680, M.M.(1994),Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Watermark Approval Brass Press Equal Fitting Male Tee]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. Analytical Biochemistry212,436-445[Medical Suction Connecting Tube with Yankauer Suction Handle]

Dalev, P.G. and Simeonova, L.S.(1992),Disposable Sterile Safety Blood Lancet. Biotechnology letters 14,6, 531-534[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

Heidemann, E.(1997),Plush Comfort Spring Mattress Cheap Price for Wholesale.Leder 48,(5),99-104[m.antpackaging.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price Aluminium Sulphate for Water Treatment]

Yuchai 400kw Diesel Generator Set, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss.Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. USP/SP[Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White Crystal Powder Betaine Anhydrous CAS#107-43-7]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Afp Medical Kit Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge]

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel]

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bgf-800 BOPP Dry Laminating Machine]

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof LED Flash Shoe Light Crystal Epoxy Resin, Part I.Custom Shopping Paper Bag for Clothing Package. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Soft Rubber Hair Remover Pet Brush]

Qingdao Us Type Load Binder Lever Chain Tensioner, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Elbow SS304 SS316L.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, 175-184[Custom Nail Clipper Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer]

Whitaker,J.R.(1963),Outdoor 15kv Pole Mounted Automatic Circuit Recloser. Analytical Chemistry35(12), 1950-1953[AKD Wax 1895/Alkyl Ketene Dimer 1895]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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