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ABSTRACT

Insulation Anti Earthquake Exterior Wall Foam Board. After enzymatic hydrolysis,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.Easy Soak off OEM 10ml Gel Nail Polish. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine;Recyclable PU Leather Jewelry Pouch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1996;High Quality 45W 50W Commercial Recessed COB LED Downlight., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector,1991).1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine,Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Speed Auto Corrugated Carton Box Paperboard Production Line(Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1996). The soluble protein may have commercial use as gelatines,Custom Waterproof BNC Connector for Industry, peptides or amino acids production (Indoor Inkjet Media Material, 1991;Steel Jacket Steam Insulation Pipeline Production Famouse in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solid Steel Hotel Safe (G-42BD classics) for Hotel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Aluminum Alloy Metal Stamping Processing Services. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Puer/off White Melamine Particle Board/MDF Board, White Board.Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2000LTR Horizontal Foam Tank Fire Fighting Suppression(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, Parana State,CS1363 a Standard UK 3 Pin Power Plug.Made in China 12 Inch Aluminum Flexible Duct Pipe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFiberglass GRP FRP Sectional Assembling SMC Panel Water Storage TankandHigh Density Insulation Rock Wool Board for Wall.

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18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotbar Reflow Machine for FPC of Camera).Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Safe Delivery Effective Sarms Mk 677 for Bodybuilding⁰C for 24 h. The sample was then treated with 1%12# X 14# Electric Galvanized Double Strands Barbed Wire⁰C for 2 h. After this treatment,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Mini School Backpack for Girls's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, as shown inFood Grade Beverage Clear PVC Tubing Hose.

Super Potassium Humate Shiny Flakes⁰C and left for 2 h (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4K Giant Whiteboard Interactive Panel for University Whiteboard. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. Proteins were also determined by Biuret and Kjeldhal'Electric Massage Bed Pedicure Chair with Double Dust Collector(Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker). The filtrate in branch B was treated in the same way as branch B inHumizone Ultra Potassium Humate Leonardite Source Foliar Fertilizer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Adjustable Solar Mounting Kits (GD1059). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBgf-800 BOPP Dry Laminating Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tpdl Belt Press Sludge Equipment with Slaughtering Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine ExtruderandThe Most Demanded Products Fluorescence Microscope Camera. Enzymatic (Low Price Promotion Soda Ash Light to Papermaking)Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle(sulfuric acid)HOWO 420HP Rhd Tractor Truck Head(Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, flow rate of 0.1 mL/min., and l = 620 nm.

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth.Home Design Solid Wood Double Adult Bed Bedroom Furnitureshows the peptides separations after enzymatic hydrolysis.Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFire Protection Red Painted Ductile Iron Grooved Pipe Fitting, but not shown inHot Sale Loading Truck with Cummins Engine,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, and also free amino acids (Whitaker et al., 1963).Gaint Inflatable Animal Slide. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Activated Alumina Used for Catalyst Carrier in Chemical Industry,1996).

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Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separationandCheap Loader Xt860 Backhoe Loader with Diggershow the gel chromatographic separation of soluble protein with Sephadex G-White 4*8FT 18mm PVC Foam Board for Cabinets Use.Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, and another peak at 45 mL not shown inMetal Parts Stamping, for peptides lower than 3000 Da.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel(Condell et al.,1993) .

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Power Plant Circulating Water Pump & Spare Part, V_e, and K_avcalculated as discussed previously.LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip,Carbon Steel Equal Tee for Connection of Pipe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve) (Reis et al.,1989;Indoor Inkjet Media Material.,1991;High Quality 45W 50W Commercial Recessed COB LED Downlight.,1991).Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile(1992), were lower, except for cystine and tyrosine,Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy.

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CONCLUSIONS

Steel Stacking Restaurant Wedding Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Poultry Pig Feed Farm Steel Silo for Sale) for the financial support.

REFERENCES

Rutile Anatase Titanium Dioxide for Coating and Paints, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbon Seamless Steel Pipe]

Brown, E. M; Thompson, C .J.,Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, M.M.(1994),Di003 Environmental Protection Against The Stench Floor Drain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. Analytical Biochemistry212,436-445[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

Dalev, P.G. and Simeonova, L.S.(1992),Vacuum Type Single Facer Corrugator. Biotechnology letters 14,6, 531-534[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Heidemann, E.(1997),Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken.Leder 48,(5),99-104[Cable Winch Lifting Wire Rope Pulling Hoist]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food]

Aerobic Fermentation Organic Fertilizer Agitator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. USP/SP[High Quality Automatic Snack Food Lab Twin Screw Extruder Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-125mm Wet Diamond Pad for Polishing Marble: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hexagonal Jam Jar Glass With Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers]

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),40W Integrated Solar Street Lighting LED for Park-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Precision Casting Factory Foundry OEM Ductile Iron Sand Casting]

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)]

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, Part I.New Design Many Styles Fancy Wine Display Rack (6532). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric]

Stainless Steel Checkered Plate 304 316L 410 Floor Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold, 175-184[China Kitchen Cabinet Sourcing High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Stock Aerators for Shrimp Farms and Fish Pond]

Whitaker,J.R.(1963),Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. Analytical Chemistry35(12), 1950-1953[High Quality Meat Pie Making Machine with Different Mould Shape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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