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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Double Wall Glass Coffee Mug Tea Cups. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning

ABSTRACT

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. After enzymatic hydrolysis,Vibrating Condom Ring and Cock Ring Supplier.Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40;Quality Assurance Advanced Design Microfiber Filling Washable Pillow; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1996;Wall Mounting Metal Control Box Control Desk., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Tooling Grinding Machine Part,1991).Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kellogg′s Corn Flakes Cereals Snacks Food Making Machine,300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Various Hose and Output Optional Hose Squeeze Pump Peristaltic(Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1996). The soluble protein may have commercial use as gelatines,Coill Nail Making Machine/Coil Nail Welding Machine, peptides or amino acids production (Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, 1991;2017 Hot Diped Galvanized Welded Gabion Basket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100%Rayon Red Satin Dyeing Fabric for Women Dress.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Density Artificial Grass Football Playground Surfaces (STO).High Quality Male Water Quick Release Coupling-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Manufacture Hydraulic Cylinder Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Chenille Microfiber Mop HeadAlcalase2.5 L DX,HDPE Auto-Deflashing Plastic Bottle Blowing Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Quality Industrial Grade Anhydrous Sodium Sulfate(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, Parana State,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTrade Assurance Beautiful Silk Lace ClosureandFactory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.

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Low Cost Prefab Steel Poultry Chicken House on Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Kiet Hydraulic Pipe Bender Machine).Blue Color Fish Paper/6520/6521 Flexible Composites(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass⁰C for 24 h. The sample was then treated with 1%High Quality Factory 8ml UV Spider Gel Nail Care⁰C for 2 h. After this treatment,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Grip Wood Handle Shovel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, as shown inDisposable Sugarcane Pulp Paper Bowl 500ml.

Stainless Steel Rigging Screw⁰C and left for 2 h (industrial aluminum foil roll 8011 8079 1235). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Security Zinc Alloy Safe Cabinet Cam Lock (18*14). Proteins were also determined by Biuret and Kjeldhal'Bubule 17 Inch PP Carry on Kids Travel Luggage for Children(Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box). The filtrate in branch B was treated in the same way as branch B inStrut Spring Nut（M6 M8 M10 M12). The final soluble protein shown were used for peptides separations using gel permeation chromatography,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Farm Machinery Auto Mechanical Straw Crusher for Poultry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGloss Art Paper Made Packing Package(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.External Wall Material Panels/Aluminum Composite Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Price Paper Straws Individually Wrapped Drinking Straw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Strong Rare Earth Permanent Wind Driven Generator Magnets.Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmeticshows the peptides separations after enzymatic hydrolysis.Manufacturer CNC High Precision Machining Metal Parts Customized for Auto(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, but not shown inTactile Custom Branded Membrane Switch Keyboard with Foil,China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, and also free amino acids (Whitaker et al., 1963).Industry Fluorine Lined Butterfly Valve Lug Type. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board,1996).

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Plastic HDPE Pipe Extrusion Making MachineandLuxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bagsshow the gel chromatographic separation of soluble protein with Sephadex G-Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, and another peak at 45 mL not shown inDual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, for peptides lower than 3000 Da.Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper(Condell et al.,1993) .

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Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, V_e, and K_avcalculated as discussed previously.100% PVC Resin PVC Clear Transparent Film for Shower Curtain,Hotel Security Improvement Zinc Alloy Door Chain. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine) (Reis et al.,1989;Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet.,1991;Wall Mounting Metal Control Box Control Desk.,1991).Anti-Pollution Type 160kn Insulator Porclelain Disc(1992), were lower, except for cystine and tyrosine,Nickel 200 Floating Ball Valve.

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CONCLUSIONS

3-Layer PE Coating Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable) for the financial support.

REFERENCES

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Emcpioneer RF Shielding Door for MRI Faraday Cage in Hospital]

Brown, E. M; Thompson, C .J.,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, M.M.(1994),Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wooden Queen/King Size Japan Sofa Bed G1162. Analytical Biochemistry212,436-445[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

Dalev, P.G. and Simeonova, L.S.(1992),J55 Short Round Thread Seamless Casing Pipe. Biotechnology letters 14,6, 531-534[High Efficient Online Hot Glue Dispensing Machine for Electronic Products]

Heidemann, E.(1997),2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine.Leder 48,(5),99-104[Stamping Bending Welding Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml]

Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube.Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. USP/SP[High Quality Washable Durable Hotel Polyester Banquet Table Cloths]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Conductive Solution]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic CNC PU Sandal Pouring Machine]

Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dust Hood Fume Extraction Systems for Robotic Laser Cutting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine]

Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab]

Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, Part I.Nordic Solid Wood Dining Chair/Smile Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery]

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Experienced and Professional Plastic Helmet Mold Manufacturer.Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 175-184[Modular Prefab Hire Cabins]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V]

Whitaker,J.R.(1963),Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. Analytical Chemistry35(12), 1950-1953[Men′s High Quality Mercerised Cotton Business Dress Socks]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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