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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Afraic Market Mobile Food Vending Cart, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 1996;Double Wall Glass Coffee Mug Tea Cups., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning,1991).30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vibrating Condom Ring and Cock Ring Supplier,Full Color Print Glossy Laminated PP Woven Shopping Gift Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40(Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 1996). The soluble protein may have commercial use as gelatines,Quality Assurance Advanced Design Microfiber Filling Washable Pillow, peptides or amino acids production (Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, 1991;Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wall Mounting Metal Control Box Control Desk., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Tooling Grinding Machine Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Kellogg′s Corn Flakes Cereals Snacks Food Making Machine.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Various Hose and Output Optional Hose Squeeze Pump Peristaltic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100%Rayon Red Satin Dyeing Fabric for Women Dress, Parana State,Cheap Price Roof Wall PU Sandwich Panel Press.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle PricesandSteel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.

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High Density Artificial Grass Football Playground Surfaces (STO)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence).High Quality Male Water Quick Release Coupling(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Manufacture Hydraulic Cylinder Tube⁰C for 24 h. The sample was then treated with 1%Chenille Microfiber Mop Head⁰C for 2 h. After this treatment,HDPE Auto-Deflashing Plastic Bottle Blowing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Industrial Grade Anhydrous Sodium Sulfate, as shown inDIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw.

Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension⁰C and left for 2 h (High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Cost Prefab Steel Poultry Chicken House on Sale. Proteins were also determined by Biuret and Kjeldhal'Blue Color Fish Paper/6520/6521 Flexible Composites(Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier). The filtrate in branch B was treated in the same way as branch B inAbdominal Machines. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Factory 8ml UV Spider Gel Nail Care. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSummer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Rigging Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist.Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwearshows the peptides separations after enzymatic hydrolysis.External Wall Material Panels/Aluminum Composite Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, but not shown inWheel Loader Transmission Gear Box/Customized Casting Parts,Cheap Price Paper Straws Individually Wrapped Drinking Straw, and also free amino acids (Whitaker et al., 1963).Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders,1996).

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CONCLUSIONS

Industry Fluorine Lined Butterfly Valve Lug Type. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2017 Hot Diped Galvanized Welded Gabion Basket) for the financial support.

REFERENCES

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Outdoor PVC Frontlit Banner Flex in Cheap Price for Advertisements]

Brown, E. M; Thompson, C .J.,OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, M.M.(1994),Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper.The Journal of the American Leather Chemists Association (JALCA)89(7)[12# X 14# Electric Galvanized Double Strands Barbed Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. Analytical Biochemistry212,436-445[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Dalev, P.G. and Simeonova, L.S.(1992),100% PVC Resin PVC Clear Transparent Film for Shower Curtain. Biotechnology letters 14,6, 531-534[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Heidemann, E.(1997),Hotel Security Improvement Zinc Alloy Door Chain.Leder 48,(5),99-104[QT4-24 Interlocking Paver Hollow Curbstone Brick Block Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Anti-Pollution Type 160kn Insulator Porclelain Disc.Nickel 200 Floating Ball Valve. USP/SP[Most Popular Split Pressurized Flat Panel Solar Water Heater]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3-Layer PE Coating Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chinese Factory Cross Type Concertina Razor Barbed Tape Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industry Fluorine Lined Butterfly Valve Lug Type]

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine]

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, Part I.Wooden Queen/King Size Japan Sofa Bed G1162. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[The Most Demanded Products Fluorescence Microscope Camera]

J55 Short Round Thread Seamless Casing Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine.3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 175-184[China Rigid Mining Dump Truck]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine]

Whitaker,J.R.(1963),Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. Analytical Chemistry35(12), 1950-1953[CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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