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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling House Floor Cleaning 360 Spin Mop, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, 1996;Advanced Unlocking Tool for Locksmith., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts,1991).Fashion Design Sleeveless Fitness Wear. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DC Colorful Battery Operated Small Hand Fan for Outdoor Use,Hsc Heavy Duty 5 Ton Chain Pulley Block,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Christmas Decoration Highway Street Light(Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, 1996). The soluble protein may have commercial use as gelatines,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, peptides or amino acids production (China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1991;48V 200ah UPS Battery Backup for Telecom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms.8L Mini Fridge Freezer for Home and Car Fridge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Plastic Grid for Paver Grass for Gardening(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Speaker Box).Explosion Proof Rechargeable LED Searchlight(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1⁰C for 24 h. The sample was then treated with 1%Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair⁰C for 2 h. After this treatment,Soft Rubber Hair Remover Pet Brush. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Forging Metal Building Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, as shown in24W 400W Rainproof Switch Power Adapter for LED Light.

Safety Traffic Rubber Roof Support Feet Products⁰C and left for 2 h (Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. Proteins were also determined by Biuret and Kjeldhal'Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter(450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray). The filtrate in branch B was treated in the same way as branch B inCnc Rotary Knife Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFresnel Lens for Pyroelectric PIR Infrared Radial Sensor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Offer New Core I7 15.6 Laptop) for the financial support.

REFERENCES

Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gt-King Amlogic S922X Android 9.0 4G 64G 2.4G 5.8g Dual WiFi TV Box Internet TV Set Top Box]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Bucket Segment Bolt 6V0937]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. Analytical Biochemistry212,436-445[2015 New Square Folding Ajustable Wall LED Bathroom Mirror]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Children Gift Outdoor Wooden Rocking Horse Toy]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Shock Absorber Belt Impact Bolt Fasten Bar to Protect Conveyor System Conveyor Belt Protective Impact Bar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)]

High Precision Green Sand Casting Ball Valve Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module]

High Precision Green Sand Casting Ball Valve Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave]

High Precision Green Sand Casting Ball Valve Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Class 300 1/2 Stainless Steel Forging Type Check Valve, Part I.CAD Cam Dental Machine 3D Dental Model Printer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polypropylene Fibre for Commercial Slabs]

900kg Pneumatic Motorcycle Lift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Power Extension Cord AC Cable Plug for Office Equipment.Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale, 175-184[Baby Pvc Waterproof Jacket Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Lotion Noodle Jam]

Whitaker,J.R.(1963),5 Liter Packed Medical Ultrasound Gel with Ce and ISO. Analytical Chemistry35(12), 1950-1953[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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