ARTICLES

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ABSTRACT

Black Powder Coated Steel Sheet Metal Housing. After enzymatic hydrolysis,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.Hot Sell 100% Seal Hexagon Glass Jam Jar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping;High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Caliber Copper Rectangular Steel Tube, 1996;2mm White Coated Paper Cake Boards Jordan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box,1991).Luxious Hotel Passenger Elevator, Titanium Elevator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yuchai 400kw Diesel Generator Set,Black Plastic GRP FRP PE Pipe Fitting for Water Supply,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2194230012 for Mercedes Benz Auto Spare Parts Brake Disc(Heavy Caliber Copper Rectangular Steel Tube, 1996). The soluble protein may have commercial use as gelatines,Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, peptides or amino acids production (Hot Sale Electrical Motors Small for Home Appliance, 1991;PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bending Shrapnel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12V Car Cigarette Lighter Adapter Power Extension Cord.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Caliber Copper Rectangular Steel Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Water Bed, Water Slide, Water Roller for Indoor Playground.Besen Portable EV Fast Charger  with Schuko Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, Parana State,Widely Use 32 Cavities Pet Preform Water Bottle Mould.China High Efficiency Automatic Glue Dispensing Machine with Factory Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heavy Caliber Copper Rectangular Steel Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCe Approval Biscuit Trayless Packing Machine (MZ-250B)andCommercial Vegetable Mushroom Coconut Meat Drying Machine.

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Cooling

CNC Machining Mechanical Parts Fabrication Service(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbon Steel Pipe ASTM A106 A53).Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall⁰C for 24 h. The sample was then treated with 1%Electronic Pulse Gas Stove Burner Igniter Transformer Parts⁰C for 2 h. After this treatment,CNC Turned Aluminum Anodized Auto Part. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Vegetable Mushroom Coconut Meat Drying Machine, as shown in3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss.

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass⁰C and left for 2 h (IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hydraulic Fold-Bend Machine Hydraulic Bending Machine. Proteins were also determined by Biuret and Kjeldhal'Delphi Control Valve 28239295 (622B)(Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player). The filtrate in branch B was treated in the same way as branch B inSmall Water Cooling Tower. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Precision Aluminum Alloy/Zinc Alloy Die Casting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBathroom Makeup LED Mirror Glass with Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White and Green Board, School Writing Board, School Furniture (GT-76C), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Current Precise LED Digital Ammeter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, V_e, and K_avcalculated as discussed previously.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module,Natural OEM Private Label Customized Skin Care Face Serum. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Safe Delivery Effective Sarms Mk 677 for Bodybuilding) (Reis et al.,1989;Hot Sale Electrical Motors Small for Home Appliance.,1991;2mm White Coated Paper Cake Boards Jordan.,1991).Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering(1992), were lower, except for cystine and tyrosine,Automatic Screw Press Dewatering Machine for Cassava Residue.

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CONCLUSIONS

Outdoor Indoor Football Soccer Goal Post Net for Kids. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Oil Wax Furniture PVC Leather (HONGJIU-498#)) for the financial support.

REFERENCES

Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Carpet Manufacturers Diamond Pattern Carpet Design for Living Room Area Carpet]

Brown, E. M; Thompson, C .J.,20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, M.M.(1994),Hot Sale High Speed Mixer Blender for Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Density Insulation Rock Wool Board for Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid. Analytical Biochemistry212,436-445[Hotbar Reflow Machine for FPC of Camera]

Dalev, P.G. and Simeonova, L.S.(1992),High Density Insulation Rock Wool Board for Wall. Biotechnology letters 14,6, 531-534[Food Grade Beverage Clear PVC Tubing Hose]

Heidemann, E.(1997),Garbage Bag Making Machine Price Trash Bag Sealing Machine.Leder 48,(5),99-104[TOE NAIL]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[110V 125V 24V Neon Indicator Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Professional Shipping Agent to Turkey-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Loading Truck with Cummins Engine]

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Surface Mounted Wall Light for Home Decoration Waterproof]

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, Part I.Crusher for Animal Waste/ Chicken Manure. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Threaded Locking Circular Connectors M16 680.Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, 175-184[60HP Industrial Water Cooled Screw Chiller for Injection Machine with Self Made Chiller Condenser Evaporator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Loader Xt860 Backhoe Loader with Digger]

Whitaker,J.R.(1963),Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. Analytical Chemistry35(12), 1950-1953[Metal Parts Stamping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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