ARTICLES

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ABSTRACT

12oz 16oz Glass Mason Jar with Handle and Straw. After enzymatic hydrolysis,Mini Freeze Dryer for Sale.5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality and Efficient Combustion UHP Graphite Electrode, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Graphite Ring Good Lubrication, 1996;Flat Head Six-Lobe with Gasket Self Tapping Screws., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw,1991).Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting,Weiying Full Computerized Plain and Invisible Sock knitting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Double Cereal Airtight Food Dispenser(Graphite Ring Good Lubrication, 1996). The soluble protein may have commercial use as gelatines,Disposable Food Packaging Paper Soup Bowl with Lids, peptides or amino acids production (500ml PP Sport Water Bottle with Ball, 1991;Stamping Fine Blanking Sheet Metal Fabrication Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Trade Assurance Beautiful Silk Lace Closure.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Needle Rollers for Bearings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Graphite Ring Good Lubrication, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heavy Duty Steel Warehouse Storage Pallet Rack.Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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14 Gauge Twist Galvanized Black Annealed Steel Wire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gas Calcined Anthracite Carburizing Agent).2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fully Automatic Digital Pid Temperature Controller⁰C for 24 h. The sample was then treated with 1%High Quality Anti-Corrosion Fiberglass Sheet and Roll⁰C for 2 h. After this treatment,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full-Automatic Folding Napkin Paper Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Frame Mount Highway Pegs Enclosure Parts, as shown in5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Group 152cm Picnic Folding in Half Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTactile Custom Branded Membrane Switch Keyboard with Foil(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

industrial aluminum foil roll 8011 8079 1235. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer) for the financial support.

REFERENCES

Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5 Buttons European Electric Hoist Type Crane Hand Push Button Switch 2 Directions Double Speeds]

Brown, E. M; Thompson, C .J.,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, M.M.(1994),Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Afraic Market Mobile Food Vending Cart. Analytical Biochemistry212,436-445[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

Dalev, P.G. and Simeonova, L.S.(1992),Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Biotechnology letters 14,6, 531-534[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

Heidemann, E.(1997),Double Wall Glass Coffee Mug Tea Cups.Leder 48,(5),99-104[Polyamide PA66 Glass Fiber 25 Pellets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. USP/SP[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vibrating Condom Ring and Cock Ring Supplier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Supplier T Shirt Design Sublimation Printing Custom Wholesale T-Shirts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple]

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Quality Assurance Advanced Design Microfiber Filling Washable Pillow, Part I.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wall Mounting Metal Control Box Control Desk.Precision Tooling Grinding Machine Part, 175-184[Hot Sale Waterproof Small Mini Children Insulated Cooler Bag Kids School Lunch Bags with Many Nice Pattern and Handle Zipper Pocket for Mother]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Trade Assurance Beautiful Silk Lace Closure]

Whitaker,J.R.(1963),Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. Analytical Chemistry35(12), 1950-1953[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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