ARTICLES

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ABSTRACT

HOWO 420HP Rhd Tractor Truck Head. After enzymatic hydrolysis,Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46;China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gaint Inflatable Animal Slide, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1996;Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel,1991).Power Plant Circulating Water Pump & Spare Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip,Carbon Steel Equal Tee for Connection of Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile(White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1996). The soluble protein may have commercial use as gelatines,Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, peptides or amino acids production (Steel Stacking Restaurant Wedding Chair, 1991;Rutile Anatase Titanium Dioxide for Coating and Paints, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Laser Cutting Machine for Sale for Die Shops From Yitai Die Making., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Di003 Environmental Protection Against The Stench Floor Drain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vacuum Type Single Facer Corrugator.Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, Parana State,Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle.New Design Many Styles Fancy Wine Display Rack (6532), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAuto Spare Parts Ignition Coil 22448-2y001andHealthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved.

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Stainless Steel Checkered Plate 304 316L 410 Floor Material(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children).Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold⁰C for 24 h. The sample was then treated with 1%Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection⁰C for 2 h. After this treatment,Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sport Anti Vibration Hiking Safety Shoes, as shown inTamper Evident Security Seals Heat Resistant Electronic Tape.

Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial⁰C and left for 2 h (PVC Wood Grain Decorative Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,24W 400W Rainproof Switch Power Adapter for LED Light. Proteins were also determined by Biuret and Kjeldhal'Industrial Threaded Plastic Connector Female Electrical Plug(Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat). The filtrate in branch B was treated in the same way as branch B inForging Automation. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGeotextile for Airport Runways/High Way, Railway(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cute Cartoon Coin Purse Children Zipper Purse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Garment Lace Coating Machine Automatic Silicone Coating Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pumpand2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980). Enzymatic (Tamper Evident Security Seals Heat Resistant Electronic Tape)South Africa Standards Solar Power Variable Message Sign LED Traffic Sign(sulfuric acid)2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin(White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels, flow rate of 0.1 mL/min., and l = 620 nm.

Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm.Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebikeshows the peptides separations after enzymatic hydrolysis.Mooncake Pastry and Plastic Packaging Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDocument Storage Durable Metal Wire Mesh Decking for Pallet Rack, but not shown inHot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes,Complete Maize Starch Wet Milling Plant with High Quality, and also free amino acids (Whitaker et al., 1963).Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (White 4*8FT 18mm PVC Foam Board for Cabinets Use,1996).

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CONCLUSIONS

Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter) for the financial support.

REFERENCES

19m Mobile LED Display Billboard for Advertising Screens, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Chrome Cast Ball Mining Anti Wear Ball]

Brown, E. M; Thompson, C .J.,Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), M.M.(1994),Replica Car Wheels with 20inch for BMW Car.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Analytical Biochemistry212,436-445[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

Dalev, P.G. and Simeonova, L.S.(1992),Cooling Tower Replaced Fill Media. Biotechnology letters 14,6, 531-534[ 400A]

Heidemann, E.(1997),A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow.Leder 48,(5),99-104[Panama Market Popular Disposable Double Layer 30g GSM Waxed Inner Paper Apple Growing Paper Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

The Most Demanded Products Fluorescence Microscope Camera, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear.Stainless Steel Expanded Mesh. USP/SP[SGS 40L Lab Use Chocolate Refiner Conching Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.ncmillingmachine.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Lf + Hf Contactless Smart Hybrid Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Lf + Hf Contactless Smart Hybrid Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Lf + Hf Contactless Smart Hybrid Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Fairy Star Light for All Kinds of Decoration Skylike, Part I.Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Linear Vibrator Screener, for Granule and Powder Selection and Classification, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.Children Hardcover Book Printing Services with Free Printing File Checking, 175-184[Sleeping Bags For Adults Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Whitaker,J.R.(1963),One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. Analytical Chemistry35(12), 1950-1953[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SwineAuthor for correspondence