ARTICLES

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ABSTRACT

Slotted Round Head Aluminum Self Tapping Screw. After enzymatic hydrolysis,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker;Hotbar Reflow Machine for FPC of Camera; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Viton Coated Half Shaft Wafer Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Claus Process Catalyst 4-6 mm, 1996;4 Different Ball Silicone Ice Cube Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Washable Durable Hotel Polyester Banquet Table Cloths,1991).16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers(Claus Process Catalyst 4-6 mm, 1996). The soluble protein may have commercial use as gelatines,Aerobic Fermentation Organic Fertilizer Agitator, peptides or amino acids production (Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, 1991;CS1363 a Standard UK 3 Pin Power Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Insulation Anti Earthquake Exterior Wall Foam Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Easy Soak off OEM 10ml Gel Nail Polish. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Claus Process Catalyst 4-6 mm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.Recyclable PU Leather Jewelry Pouch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, Parana State,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Claus Process Catalyst 4-6 mm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlush Comfort Spring Mattress Cheap Price for WholesaleandSpecial Shape Spring with Good Quality and Fast Delivery.

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High Speed Auto Corrugated Carton Box Paperboard Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office).Custom Waterproof BNC Connector for Industry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Indoor Inkjet Media Material⁰C for 24 h. The sample was then treated with 1%Steel Jacket Steam Insulation Pipeline Production Famouse in China⁰C for 2 h. After this treatment,Solid Steel Hotel Safe (G-42BD classics) for Hotel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Aluminum Alloy Metal Stamping Processing Services, as shown inChina Factory Organic Ethyl Ascorbic Acid.

Puer/off White Melamine Particle Board/MDF Board, White Board⁰C and left for 2 h (316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2000LTR Horizontal Foam Tank Fire Fighting Suppression. Proteins were also determined by Biuret and Kjeldhal'High Spped Weaving Machine 6(Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women). The filtrate in branch B was treated in the same way as branch B inBig Tank Portable Evaporative Air Cooler/ Water Cooler/ Desert Cooler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Poultry Pig Feed Farm Steel Silo for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnimal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Made in China 12 Inch Aluminum Flexible Duct Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Super Potassium Humate Shiny Flakes.10mm Cubes N52 NdFeB Block Magnets for Electronic Componentsshows the peptides separations after enzymatic hydrolysis.4K Giant Whiteboard Interactive Panel for University Whiteboard(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, but not shown inStainless Steel CF8/CF8m Street Elbow Pipe Fittings,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, and also free amino acids (Whitaker et al., 1963).Electric Massage Bed Pedicure Chair with Double Dust Collector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Claus Process Catalyst 4-6 mm,1996).

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Test-630 Automatic Protective Relay Tester, V_e, and K_avcalculated as discussed previously.Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle,Tpdl Belt Press Sludge Equipment with Slaughtering Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net) (Reis et al.,1989;Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment.,1991;4 Different Ball Silicone Ice Cube Maker.,1991).3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)(1992), were lower, except for cystine and tyrosine,The Most Demanded Products Fluorescence Microscope Camera.

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CONCLUSIONS

Low Price Promotion Soda Ash Light to Papermaking. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector) for the financial support.

REFERENCES

Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5mm Led Diodes 12v]

Brown, E. M; Thompson, C .J.,HOWO 420HP Rhd Tractor Truck Head, M.M.(1994),Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.The Journal of the American Leather Chemists Association (JALCA)89(7)[Extra Heavy Wall Thickness Seamless Tubes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. Analytical Biochemistry212,436-445[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. Biotechnology letters 14,6, 531-534[Shengya Steel Concrete Electric Pole Mould]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Travel Refillable Perfume Atomizer Spray Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Phase Digital Electric Power Meter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Air Cooler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Power Plant Circulating Water Pump & Spare Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, Part I.Carbon Steel Equal Tee for Connection of Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Grip Wood Handle Shovel]

Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy.Steel Stacking Restaurant Wedding Chair, 175-184[Backhoe Loaders Price In India Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Whitaker,J.R.(1963),Rutile Anatase Titanium Dioxide for Coating and Paints. Analytical Chemistry35(12), 1950-1953[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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