ARTICLES

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ABSTRACT

Industrial Sausage Meat Smoker Oven/Smokerhouse Oven. After enzymatic hydrolysis,Men′s High Quality Mercerised Cotton Business Dress Socks.Livining Room Rugs Geometric Patterns Carpets 6′*9′. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lamella Clarifier for Wastewater Treatment Plant;Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), 1996;2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9,1991).E6000 Handheld Three Phase Power Quality and Energy Analyzer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Qg Jg Fg Series Air Dryer Equipment Factory,Motor Bearing 6014 2RS Deep Groove Ball Bearing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line(Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), 1996). The soluble protein may have commercial use as gelatines,Pet Short Fiber Non Woven Geotextile for Landfill Filtration, peptides or amino acids production (Pre Painted Steel Coil and Sheet, 1991;Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Maleic Anhydride CAS 108-31-6.Plastic HDPE Pipe Extrusion Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jaguar Jb-390 Automatic PCB Loader and Unloader(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pneumatic Cut off Ball Valve with Flange Thread Weld End, Parana State,Chinese Color Steel Glazed Tile Purlin Roll Forming Machine.Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft LidandSurgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy.

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Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank).Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Decorative Aluminum Wall Cladding Panel with Durable Painting Finish⁰C for 24 h. The sample was then treated with 1%Steel Grating for Industrial Platform⁰C for 2 h. After this treatment,50Hz/60Hz Low Frequency Home Power Inverter 5000W. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, as shown inManufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre.

Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c⁰C and left for 2 h (Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag. Proteins were also determined by Biuret and Kjeldhal'Lint Free Nail Gel Polish Remover Nonwoven Wipes(Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes). The filtrate in branch B was treated in the same way as branch B inGuangzhou Flk Bottle Filler Canning Jars Filling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shs250X500 Galvanized Square Rectangular Steel Tube Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Double Hole ABS Plastic CNC Machined Parts Importer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/KZ-19/16/13 Pneumatic Combination Steel Binding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

DMD Polyester Film/6630/Electric Insulation PaperandMedical Packing Self Sealing Sterilization Pouch. Enzymatic (Water Filter Cartridge PP Sediment Filter Cartridge)Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale(sulfuric acid)Disposable Skin Stapler (CSPF-35W)(Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), flow rate of 0.1 mL/min., and l = 620 nm.

New Customized Mug Heat Press Machine Price.Small Poultry Pig Feed Farm Steel Silo for Saleshows the peptides separations after enzymatic hydrolysis.All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, but not shown in1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve,Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, and also free amino acids (Whitaker et al., 1963).Shengya Steel Concrete Electric Pole Mould. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S),1996).

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China Manufacturer Garbage Incinerator, V_e, and K_avcalculated as discussed previously.China water meter factory water flow meter vane wheel,4 Heads High Speed Professional Paper Cardboard Laser Cutter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electric Winch With Groove Drum) (Reis et al.,1989;Pre Painted Steel Coil and Sheet.,1991;2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market.,1991).Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll(1992), were lower, except for cystine and tyrosine,Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved.

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CONCLUSIONS

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluno Aluminum Veneer Price Aluminium Curtain Wall Details) for the financial support.

REFERENCES

Gas Calcined Anthracite Carburizing Agent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[School Sport Gym Sack Pouch Backpack Drawstring Bag]

Brown, E. M; Thompson, C .J.,Silver Hammered Round Ball and Natural Stone Necklace, M.M.(1994),OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape.The Journal of the American Leather Chemists Association (JALCA)89(7)[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies. Analytical Biochemistry212,436-445[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

Dalev, P.G. and Simeonova, L.S.(1992),12 Teeth Green Painting Steel Rake. Biotechnology letters 14,6, 531-534[Fast Install Polyurethane Decorative Wall Panel for Homes]

Heidemann, E.(1997),Car Parking or Access Control 2.4 GHz Active RFID Card.Leder 48,(5),99-104[High Quality VFD Single Phase AC Motor Frequency Inverter China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4lz-4.5 Small Tank Kubota Similar Rice Harvester: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tec Thermoelectric Cooling Module Air Conditioner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

High Quality Automatic Snack Food Lab Twin Screw Extruder Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

High Quality Automatic Snack Food Lab Twin Screw Extruder Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

High Quality Automatic Snack Food Lab Twin Screw Extruder Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IC LED Wire Loop Height Measuring Microscope, Part I.Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Whisky Chilling Stones.Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove, 175-184[Stainless Steel Straws Cleaner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Whitaker,J.R.(1963),CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. Analytical Chemistry35(12), 1950-1953[Threaded Locking Circular Connectors M16 680]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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