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ABSTRACT

High Quality Needle Rollers for Bearings. After enzymatic hydrolysis,Heavy Duty Steel Warehouse Storage Pallet Rack.Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1996;Meet All Industry Needs with Openly Design High Temperature Oven., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (380V 3.0HP Peristaltic Pump Swimming Pool Pump,1991).Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,14 Gauge Twist Galvanized Black Annealed Steel Wire,2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fully Automatic Digital Pid Temperature Controller(PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1996). The soluble protein may have commercial use as gelatines,High Quality Anti-Corrosion Fiberglass Sheet and Roll, peptides or amino acids production (Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, 1991;Full-Automatic Folding Napkin Paper Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Frame Mount Highway Pegs Enclosure Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10mm Tempered Bathroom Glass Safety Glass for Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet.Cattle Feed Factory Used Dust Collector and Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Australia Two Pins Power Cord with SAA Certification(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal).87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%⁰C for 24 h. The sample was then treated with 1%Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net⁰C for 2 h. After this treatment,Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pickled Cucumber Production Line/Making Machine, as shown inHot Double Cereal Airtight Food Dispenser.

Rectangular Lunch Tin Box with Handle⁰C and left for 2 h (High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Inflatable Cheering Stick for Promotional Gift. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. Proteins were also determined by Biuret and Kjeldhal'Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts). The filtrate in branch B was treated in the same way as branch B inSofa Printing Paper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Price Roof Wall PU Sandwich Panel Press⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, pH 3.25., mobile phase B: buffer solution of 0.01 mol/industrial aluminum foil roll 8011 8079 1235, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

200m Range Video DVR Imagers for Hunting Digital Infrared Night VisionandHospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. Enzymatic (High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket)Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet(sulfuric acid)Afraic Market Mobile Food Vending Cart(PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, flow rate of 0.1 mL/min., and l = 620 nm.

Double Wall Glass Coffee Mug Tea Cups.High Quality Hydraulic Cylinder Tubes for Hydraulic Cylindershows the peptides separations after enzymatic hydrolysis.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, but not shown inMade in China 12 Inch Aluminum Flexible Duct Pipe,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, and also free amino acids (Whitaker et al., 1963).Vibrating Condom Ring and Cock Ring Supplier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer,1996).

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CONCLUSIONS

Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm) for the financial support.

REFERENCES

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Building Material 304 Stainless Steel Coil with 2b/Ba Surface Finish]

Brown, E. M; Thompson, C .J.,Various Hose and Output Optional Hose Squeeze Pump Peristaltic, M.M.(1994),Coill Nail Making Machine/Coil Nail Welding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wire Mesh Welding Size 10mm X 10mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Gifts Customized Woven Polyester Lanyard for VIP Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. Analytical Biochemistry212,436-445[PA Flexible Plastic Corrugated Loom Tubing]

Dalev, P.G. and Simeonova, L.S.(1992),2017 Hot Diped Galvanized Welded Gabion Basket. Biotechnology letters 14,6, 531-534[1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll]

Heidemann, E.(1997),Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash.Leder 48,(5),99-104[5.5hp Forward Plate Compactor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AISI 430 Ba/2b Embossed Stainless Steel Sheet]

Stamping Fine Blanking Sheet Metal Fabrication Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),100%Rayon Red Satin Dyeing Fabric for Women Dress.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. USP/SP[Abstract Light and Shadow Metal Oil Painting for Deco]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Density Artificial Grass Football Playground Surfaces (STO): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cute Cartoon Coin Purse Children Zipper Purse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pneumatic Air Leg Rock Drill]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Construction Material Hoist Rack and Pinion]

High Quality Male Water Quick Release Coupling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Manufacture Hydraulic Cylinder Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb]

High Quality Male Water Quick Release Coupling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sc Fiber Optic Fast Connector]

High Quality Male Water Quick Release Coupling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chenille Microfiber Mop Head, Part I.HDPE Auto-Deflashing Plastic Bottle Blowing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Spherical Plain Radical Bearings]

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Industrial Grade Anhydrous Sodium Sulfate.Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, 175-184[Flat Metal Roof Sheets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Carbon Steel 20g Gr. B Steam Cooling Fin Tube]

Whitaker,J.R.(1963),316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. Analytical Chemistry35(12), 1950-1953[China Factory Price Hg1 Self Adjustable Pipe Tank Rotator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Puer/off White Melamine Particle Board/MDF BoardAuthor for correspondence