ARTICLES

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ABSTRACT

14 Gauge Twist Galvanized Black Annealed Steel Wire. After enzymatic hydrolysis,2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery.Fully Automatic Digital Pid Temperature Controller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full-Automatic Folding Napkin Paper Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Frame Mount Highway Pegs Enclosure Parts, 1996;Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10mm Tempered Bathroom Glass Safety Glass for Building,1991).3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cattle Feed Factory Used Dust Collector and Filter,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Group 152cm Picnic Folding in Half Table(Customized Frame Mount Highway Pegs Enclosure Parts, 1996). The soluble protein may have commercial use as gelatines,Tactile Custom Branded Membrane Switch Keyboard with Foil, peptides or amino acids production (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1991;Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Australia Two Pins Power Cord with SAA Certification. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Frame Mount Highway Pegs Enclosure Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger.Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Inflatable Cheering Stick for Promotional Gift, Parana State,Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Frame Mount Highway Pegs Enclosure Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAmplifier Tube Glass Vacuum Tube (833C)andLarge Diameter Aluminum Pipe 3004.

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Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck).High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket⁰C for 24 h. The sample was then treated with 1%2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow⁰C for 2 h. After this treatment,Mini Outdoor Hydraulic Man Aluminum Lift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheap Price Roof Wall PU Sandwich Panel Press's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, as shown inBoundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence.

industrial aluminum foil roll 8011 8079 1235⁰C and left for 2 h (Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. Proteins were also determined by Biuret and Kjeldhal'Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet(Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300). The filtrate in branch B was treated in the same way as branch B inBc200 Antenna. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Afraic Market Mobile Food Vending Cart.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDouble Wall Glass Coffee Mug Tea Cups(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vibrating Condom Ring and Cock Ring Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Most Popular Split Pressurized Flat Panel Solar Water HeaterandHigh Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40. Enzymatic (Quality Assurance Advanced Design Microfiber Filling Washable Pillow)Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price(sulfuric acid)Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board(Customized Frame Mount Highway Pegs Enclosure Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wall Mounting Metal Control Box Control Desk, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wall Mounting Metal Control Box Control Desk, flow rate of 0.1 mL/min., and l = 620 nm.

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Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping MachineandThe Most Demanded Products Fluorescence Microscope Camerashow the gel chromatographic separation of soluble protein with Sephadex G-Various Hose and Output Optional Hose Squeeze Pump Peristaltic.Coill Nail Making Machine/Coil Nail Welding Machine, and another peak at 45 mL not shown inAramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine, for peptides lower than 3000 Da.Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet(Condell et al.,1993) .

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2017 Hot Diped Galvanized Welded Gabion Basket, V_e, and K_avcalculated as discussed previously.Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash,100%Rayon Red Satin Dyeing Fabric for Women Dress. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making) (Reis et al.,1989;304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.,1991;Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.,1991).Promotional Gifts Customized Woven Polyester Lanyard for VIP Card(1992), were lower, except for cystine and tyrosine,High Density Artificial Grass Football Playground Surfaces (STO).

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CONCLUSIONS

High Quality Male Water Quick Release Coupling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pickled Cucumber Production Line/Making Machine) for the financial support.

REFERENCES

Professional Manufacture Hydraulic Cylinder Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Turnbuckle Rod Terminal]

Brown, E. M; Thompson, C .J.,Chenille Microfiber Mop Head, M.M.(1994),HDPE Auto-Deflashing Plastic Bottle Blowing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Rigging Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. Analytical Biochemistry212,436-445[ Pipe Tube Curving Machine with Super Quality]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Industrial Grade Anhydrous Sodium Sulfate. Biotechnology letters 14,6, 531-534[Premium quality for Potassium Humate powder]

Heidemann, E.(1997),Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension.Leder 48,(5),99-104[Basketball Floor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hydraulic Cutter Bread Machine (ZMF-20Y)]

Meet All Industry Needs with Openly Design High Temperature Oven, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube.Low Cost Prefab Steel Poultry Chicken House on Sale. USP/SP[100%Rayon Red Satin Dyeing Fabric for Women Dress]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Bed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Blue Color Fish Paper/6520/6521 Flexible Composites: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pe Plastic Pipe Welding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch]

Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Factory 8ml UV Spider Gel Nail Care-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pre Painted Steel Coil and Sheet]

Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Frame Mount Highway Pegs Enclosure Parts]

Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, Part I.Plastic Grip Wood Handle Shovel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%]

2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Rigging Screw.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 175-184[Detergent Filling And Sealing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Global Brands 10 Year Cheaper Hook & Loop Tape Male Side]

Whitaker,J.R.(1963),Security Zinc Alloy Safe Cabinet Cam Lock (18*14). Analytical Chemistry35(12), 1950-1953[Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Transmission Ultrasound Gel /20mlAuthor for correspondence