ARTICLES

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ABSTRACT

Aluminium Profile Bending Machine for Insulating Glass. After enzymatic hydrolysis,Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws.304 Thick Wall Pressure Vessel Heat Exchanger Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Extra Heavy Wall Thickness Seamless Tubes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shrink Sleeves Labels for Packaging Labeling, 1996;IP65 Canopy LED Light Fixture 150W LED Gas Station Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Mould Dongguan Molding Factory,1991).OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004,Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles(Shrink Sleeves Labels for Packaging Labeling, 1996). The soluble protein may have commercial use as gelatines,Good Quality Banded Type Tee, peptides or amino acids production (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1991;Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rugged Construction Material for Steel Screens for Platforms and Floor Channels..

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FKM Rubber Gasket/Rubber O Rings in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shrink Sleeves Labels for Packaging Labeling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Inclined Waste Water Treatment Lamella Clarifier.33kv 100kVA Dry-Type Distribution Transformer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PVC Corrugated or Smooth Surface Helix Suction Water Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wall Mounting Metal Control Box Control Desk).Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ivd Component PP Barbed Threaded Connector⁰C for 24 h. The sample was then treated with 1%Workshop Tools 7.5 Ton Electric Winch with Dual Speed⁰C for 2 h. After this treatment,Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, as shown inHotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Efficient Online Hot Glue Dispensing Machine for Electronic Products(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automotive Sheet Metal Stamping Car Auto Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Etched Stainless Steel Titanium SheetandIndoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable. Enzymatic (2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1)Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation(sulfuric acid)New Patient Hoist Lift(Shrink Sleeves Labels for Packaging Labeling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), flow rate of 0.1 mL/min., and l = 620 nm.

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine.CNC Turned Aluminum Anodized Auto Partshows the peptides separations after enzymatic hydrolysis.Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMl33-2mm Eddy Current Linear Position/Displacement Sensor, but not shown inColor Change LED Restaurant Dinner Chair and Table Restaurant Furniture,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng, and also free amino acids (Whitaker et al., 1963).0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shrink Sleeves Labels for Packaging Labeling,1996).

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High Quality Bucket Segment Bolt 6V0937, V_e, and K_avcalculated as discussed previously.Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture,Outdoor Server Rack Network Data Cabinet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Weiying Full Computerized Plain and Invisible Sock knitting Machine) (Reis et al.,1989;Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.,1991;IP65 Canopy LED Light Fixture 150W LED Gas Station Light.,1991).Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera(1992), were lower, except for cystine and tyrosine,Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House.

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CONCLUSIONS

High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)) for the financial support.

REFERENCES

Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2015 New Square Folding Ajustable Wall LED Bathroom Mirror]

Brown, E. M; Thompson, C .J.,OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, M.M.(1994),Carbon Steel Pipe ASTM A106 A53.The Journal of the American Leather Chemists Association (JALCA)89(7)[ for Granule and Powder Selection and Classification]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. Analytical Biochemistry212,436-445[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

Dalev, P.G. and Simeonova, L.S.(1992),Peptides Powder Ghrp 2 for Muscle Building with Super Quality. Biotechnology letters 14,6, 531-534[Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c]

Heidemann, E.(1997),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.Leder 48,(5),99-104[Pump Body]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings]

Wood Fireplace Double Skin Chimney Flue Pipes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),EPDM, PVC Car or Door Rubber Sealing Strip.Black Color Tube Eyelet Lift Gas Spring. USP/SP[Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Safety Edge Sensor for Agv Automated Guided Vehicle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Fashion Diaper Bags Mummy Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Indoor Kids Ride on Animal Toys Kids′ Plastic Rocking Horse]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike]

Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Design Felt Belt Type Cooling Table/Handling Extrusion System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Recycling Centrifugal Dewatering Machine]

Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood, Part I.Ycom USB Charger and Data Cable with Aluminum for Type C. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mosquito Proof Fiberglass Fly Screen]

Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input.Seashell Special Shape Magnetic Eye Shadow Empty Palette, 175-184[fastlinepcb]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade Ivory Paper Cupcake Boxes]

Whitaker,J.R.(1963),on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage. Analytical Chemistry35(12), 1950-1953[ Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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