ARTICLES

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ABSTRACT

Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. After enzymatic hydrolysis,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating.Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Single Sphere Rubber Expansion Joint;Cold Press Circular Wood Cutting Machine Blade; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Trench Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1996;Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial & Household Electrical Wiring Harness for Water Dispensers,1991).Wicker Flower Baskets for Planter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Engine Parts Suspension Control Arm OEM 54501-Jg00b(Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1996). The soluble protein may have commercial use as gelatines,Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, peptides or amino acids production (Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker, 1991;Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Thickness EVA High Density Foam EVA Roll.Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbide Mould Parts Moulding Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, Parana State,Abrasive 6 Sanding Disc for Automotive Industry.Electric Furniture Lift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)andPipe Connection Types of Pressing Scaffolding Universal Clamp.

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Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow).Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Construction Material Hoist Rack and Pinion⁰C for 24 h. The sample was then treated with 1%3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out⁰C for 2 h. After this treatment,Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses, as shown in500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle.

High Quality Endoscopic Surgical Adult Disposable Bite Block⁰C and left for 2 h (Australia Two Pins Power Cord with SAA Certification). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China OEM ABS Clear Plastic Injection Molding. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Widely Use 32 Cavities Pet Preform Water Bottle Mould. Proteins were also determined by Biuret and Kjeldhal'Black Powder Coated Steel Sheet Metal Housing(5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber). The filtrate in branch B was treated in the same way as branch B inPrecision Aluminum and ABS CNC Rapid Prototyping. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sell 100% Seal Hexagon Glass Jam Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Caliber Copper Rectangular Steel Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2mm White Coated Paper Cake Boards Jordan, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Precision Casting Factory Foundry OEM Ductile Iron Sand Casting.Disposable Food Packaging Paper Soup Bowl with Lidsshows the peptides separations after enzymatic hydrolysis.Hot Sale Electrical Motors Small for Home Appliance(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommercial & Household Electrical Wiring Harness for Water Dispensers, but not shown in2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, and also free amino acids (Whitaker et al., 1963).Bending Shrapnel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses,1996).

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CONCLUSIONS

Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder) for the financial support.

REFERENCES

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Advertising Led Display Screen]

Brown, E. M; Thompson, C .J.,China High Efficiency Automatic Glue Dispensing Machine with Factory Price, M.M.(1994),CNC Machining Mechanical Parts Fabrication Service.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheapest Container Living House/Office/Kiosk]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. Analytical Biochemistry212,436-445[High Frequency Online UPS W/3W-20K]

Dalev, P.G. and Simeonova, L.S.(1992),Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Biotechnology letters 14,6, 531-534[Yet905 Wireless Keypad Doorbell Kits with Remote Control]

Heidemann, E.(1997),Electronic Pulse Gas Stove Burner Igniter Transformer Parts.Leder 48,(5),99-104[Laparoscope 10mm 30 Degrees]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling]

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Turned Aluminum Anodized Auto Part.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator. USP/SP[Integrated Waterproof PVC Lamination Bathroom Wall Panels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Commercial Vegetable Mushroom Coconut Meat Drying Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Changchuan 6kw Air Cooled Electro Spindle Motor Mhs60-6018fs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[355nm UV Laser Marking Equipment for Crystal Materials]

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Headband Big Headphone EVA Bag Tool Case Specialized Storage Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EAS Anti-Theft Optical Security Tag for Glasses Bottle]

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware]

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Fold-Bend Machine Hydraulic Bending Machine, Part I.Delphi Control Valve 28239295 (622B). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain]

OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers.Bathroom Makeup LED Mirror Glass with Light, 175-184[Sublimation 2d Phone Case]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Women′s Seamless Strapless Bra]

Whitaker,J.R.(1963),Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence. Analytical Chemistry35(12), 1950-1953[Cable Length 11 Gun Safety Padlock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

J55 Short Round Thread Seamless Casing PipeAuthor for correspondence