ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment

ABSTRACT

Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification. After enzymatic hydrolysis,Programmable Salt Spray Test Chamber for Corrosion Testing/Test.Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FKM Bearing Oil Seal /Customized;Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, 1996;Max Twintier Rb441t Using Wire Coils Tw1061t., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm,1991).Manufacturer Supply Durable Grey Color Non-Slip EVA Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Feed Storage OEM Grain Storage Silo 1cbm to 200cbm,Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Captain Woodwork Sanding Machine/Sanding Table(Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, 1996). The soluble protein may have commercial use as gelatines,Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, peptides or amino acids production (Atg39 100W LED Flood Light Wall Light Wall Washer, 1991;China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gold Inlaid 24K Gold / Flowers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board.Hydraulic Cutter Bread Machine (ZMF-20Y)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels).Ceiling Mounted IV Stand Stainlesss Steel I V Pole(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pipe Connection Types of Pressing Scaffolding Universal Clamp⁰C for 24 h. The sample was then treated with 1%Ml33-2mm Eddy Current Linear Position/Displacement Sensor⁰C for 2 h. After this treatment,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Long Lasting Custom Private Label Glitter Lip Gloss, as shown inLf + Hf Contactless Smart Hybrid Card.

ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable⁰C and left for 2 h (High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School. Proteins were also determined by Biuret and Kjeldhal'High Quality Dyed Colored Sand, Natural Coloured Sand(Small Portable Scooters Folding Electric Scooters for Adults). The filtrate in branch B was treated in the same way as branch B inThe Stretch Film. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Various Bridge NdFeB Motor Magnets with High Performance. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite Melamine Powder for Industrial Uses(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PE Film Blowing Machine (SJ-1000-1300-1500), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Selling House Floor Cleaning 360 Spin MopandEco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag. Enzymatic (304 304L 904L 309S 310S Stainless Steel Design Checkered Plate)High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass(sulfuric acid)2017 Factory Directly Oversized Women Sunglasses(Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quanlity PTFE Thread Seal Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quanlity PTFE Thread Seal Tape, flow rate of 0.1 mL/min., and l = 620 nm.

Fiberglass Grating, FRP Grating, GRP Grating.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracketshows the peptides separations after enzymatic hydrolysis.Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube, but not shown inCustomized Stainless Steel Door Lock Strike Plate Metal Lock Bracket,Rutile Titanium Dioxide Exterior Emulsion Paint, and also free amino acids (Whitaker et al., 1963).Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce,1996).

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Tree Root Grinder Stump GrinderandStainless Steel Whisky Chilling Stonesshow the gel chromatographic separation of soluble protein with Sephadex G-Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines.Stainless Steel Hole Punching Machine/ CNC Perforation Machine, and another peak at 45 mL not shown inSteel Grating for Industrial Platform, for peptides lower than 3000 Da.Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube(Condell et al.,1993) .

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Pet and PP Geobag with UV High Strength Thermal, V_e, and K_avcalculated as discussed previously.3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF,Magnetic Latching Relay for Health Life. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll) (Reis et al.,1989;Atg39 100W LED Flood Light Wall Light Wall Washer.,1991;Max Twintier Rb441t Using Wire Coils Tw1061t.,1991).More Cavities Plastic Mould Design(1992), were lower, except for cystine and tyrosine,Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300.

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CONCLUSIONS

High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042) for the financial support.

REFERENCES

Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[VIA]

Brown, E. M; Thompson, C .J.,Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, M.M.(1994),High Current Temperature Rising Test Large Current Temperature Rising Test System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Black Steel ASTM A192 Seamless Pipe Sch120]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Animal Manure Fertilizer Fermentation Machine. Analytical Biochemistry212,436-445[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Dalev, P.G. and Simeonova, L.S.(1992),Compact New Design IP65 Toggle Limit Switch. Biotechnology letters 14,6, 531-534[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

Heidemann, E.(1997),Teflon for Industrial Plastic Gasket Seal with Good Quality.Leder 48,(5),99-104[Customized Grey Iron Sand Casting Durable Ductile Iron Pump Body]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Compact New Design IP65 Toggle Limit Switch]

V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Double Walled Stainless Steel Insulated Water Bottle.Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass. USP/SP[PE Film Blowing Machine (SJ-1000-1300-1500)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbon Steel Equal Tee for Connection of Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2018 Cheap PU Leather Bracelet in Alloy Jewelry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rutile Titanium Dioxide Exterior Emulsion Paint]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gummy Bear Candy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Max Twintier Rb441t Using Wire Coils Tw1061t]

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Competetive Price Tension Springs for Stapler, Part I.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Factory Offer New Core I7 15.6 Laptop]

Men Casual Short Pants Custom Quality Running Sports Wear Shorts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Horizontal Window Shade Blind Zebra Dual Roller Blinds Day.New Fashion Diaper Bags Mummy Bag, 175-184[Bike Ceramic Bearings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

Whitaker,J.R.(1963),Mellow OEM Plastic Pet House Ml-HP004. Analytical Chemistry35(12), 1950-1953[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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