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ABSTRACT

Steel Grating for Industrial Platform. After enzymatic hydrolysis,50Hz/60Hz Low Frequency Home Power Inverter 5000W.ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, 1996;Lint Free Nail Gel Polish Remover Nonwoven Wipes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shs250X500 Galvanized Square Rectangular Steel Tube Price,1991).Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die(3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, 1996). The soluble protein may have commercial use as gelatines,Mini Double Hole ABS Plastic CNC Machined Parts Importer, peptides or amino acids production (KZ-19/16/13 Pneumatic Combination Steel Binding Machine, 1991;Medical Packing Self Sealing Sterilization Pouch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Filter Cartridge PP Sediment Filter Cartridge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Disposable Skin Stapler (CSPF-35W). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827).New Customized Mug Heat Press Machine Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China water meter factory water flow meter vane wheel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm).4 Heads High Speed Professional Paper Cardboard Laser Cutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll⁰C for 24 h. The sample was then treated with 1%Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved⁰C for 2 h. After this treatment,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gas Calcined Anthracite Carburizing Agent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silver Hammered Round Ball and Natural Stone Necklace, as shown inAfraic Market Mobile Food Vending Cart.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4lz-4.5 Small Tank Kubota Similar Rice Harvester(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/IC LED Wire Loop Height Measuring Microscope, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, V_e, and K_avcalculated as discussed previously.EAS Anti-Theft Optical Security Tag for Glasses Bottle,Single Phase Digital Electric Power Meter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( School Furniture (GT-76C)) (Reis et al.,1989;KZ-19/16/13 Pneumatic Combination Steel Binding Machine.,1991;Lint Free Nail Gel Polish Remover Nonwoven Wipes.,1991).Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly(1992), were lower, except for cystine and tyrosine,2W 100lm LED Solar Lawn Light for Garden or Yard Lighting.

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CONCLUSIONS

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker) for the financial support.

REFERENCES

28mm China High Quality Supplier Screw Cannon Cap for Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10ml bottle wholesale]

Brown, E. M; Thompson, C .J.,Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, M.M.(1994),Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure.The Journal of the American Leather Chemists Association (JALCA)89(7)[Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nonwovenface Mask Production Line. Analytical Biochemistry212,436-445[3D Good Quality Laser Engraving Machine for Souvenirs Shop]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Professional Indoor&Outdoor Trampoline Park. Biotechnology letters 14,6, 531-534[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cleaning Lint Free Microfiber Mop]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbide Spray Nozzle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

High Output Vulcanizing Press Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solar Panel PV System Mc4 Circular Connector]

High Output Vulcanizing Press Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Silver Hammered Round Ball and Natural Stone Necklace]

High Output Vulcanizing Press Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, Part I.Transmission Ultrasound Gel /20ml, 250ml, 5L. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence.Food Grade Ivory Paper Cupcake Boxes, 175-184[m.miracle-leather-bag.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Whitaker,J.R.(1963),Slide Switch - DC Power Jack RoHS. Analytical Chemistry35(12), 1950-1953[LED Emergency Exit Light Rechargeable 220V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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