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ABSTRACT

Bgf-800 BOPP Dry Laminating Machine. After enzymatic hydrolysis,Test-630 Automatic Protective Relay Tester.Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tpdl Belt Press Sludge Equipment with Slaughtering Machine;3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.The Most Demanded Products Fluorescence Microscope Camera, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Price Promotion Soda Ash Light to Papermaking, 1996;Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (HOWO 420HP Rhd Tractor Truck Head,1991).Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth,Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb(Low Price Promotion Soda Ash Light to Papermaking, 1996). The soluble protein may have commercial use as gelatines,Gaint Inflatable Animal Slide, peptides or amino acids production (White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1991;Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Power Plant Circulating Water Pump & Spare Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Price Promotion Soda Ash Light to Papermaking, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carbon Steel Equal Tee for Connection of Pipe.Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, Parana State,Adjustable Solar Mounting Kits (GD1059).Vacuum Type Single Facer Corrugator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Low Price Promotion Soda Ash Light to Papermaking, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Factory 8ml UV Spider Gel Nail CareandHome Appliance Blender Juicer Motor.

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Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotel Security Improvement Zinc Alloy Door Chain).OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve⁰C for 24 h. The sample was then treated with 1%125mm Wet Diamond Pad for Polishing Marble⁰C for 2 h. After this treatment,Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)40W Integrated Solar Street Lighting LED for Park's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, as shown in33kv 100kVA Dry-Type Distribution Transformer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/24W 400W Rainproof Switch Power Adapter for LED Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Zinc Coated Galvanized WireandIndustrial Threaded Plastic Connector Female Electrical Plug. Enzymatic (Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery)Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity(sulfuric acid)Geotextile for Airport Runways/High Way, Railway(Low Price Promotion Soda Ash Light to Papermaking, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), flow rate of 0.1 mL/min., and l = 620 nm.

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture.50kg Soya Bean Seed PP Woven Bags Sacksshows the peptides separations after enzymatic hydrolysis.Cute Cartoon Coin Purse Children Zipper Purse(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle Cylinder Mini ATV with EPA Certificate (MDL GA003-2), but not shown inBlack Powder Coated Steel Sheet Metal Housing,Garment Lace Coating Machine Automatic Silicone Coating Machine, and also free amino acids (Whitaker et al., 1963).2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Price Promotion Soda Ash Light to Papermaking,1996).

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CONCLUSIONS

Top Furniture Cheap Iron Stacking Banquet Hall Chairs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making) for the financial support.

REFERENCES

Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Prefab Steel Carport Warehouse Tent Metal Storage Shed (TW732J)]

Brown, E. M; Thompson, C .J.,Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, M.M.(1994),High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbon Steel Round/Square Pipe Tube Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. Analytical Biochemistry212,436-445[1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. Biotechnology letters 14,6, 531-534[Single Spindle Micro CNC Lathe Ck0640]

Heidemann, E.(1997),Mosquito Proof Fiberglass Fly Screen.Leder 48,(5),99-104[Air Compressor Nebulizer Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FKM Bearing Oil Seal /Customized]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vacuum Type Single Facer Corrugator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-19m Mobile LED Display Billboard for Advertising Screens: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove]

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Replica Car Wheels with 20inch for BMW Car-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, Part I.Cooling Tower Replaced Fill Media. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear.Stainless Steel Expanded Mesh, 175-184[Egg Carton Box Packing Machine High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Explosion Proof Rechargeable LED Searchlight]

Whitaker,J.R.(1963),Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2. Analytical Chemistry35(12), 1950-1953[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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