ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Single-Screw Plastic Extruders Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube

ABSTRACT

High Quanlity Colorful Aluminum Spacer/Standoff. After enzymatic hydrolysis,5g OPP Silica Gel Desiccant.Electric Winch With Groove Drum. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump;Truck Air Dryer cartridge 14bar; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3mm White Waterproof PVC Foam Board, 1996;Educational Equipment Smart Multi Touch Interactive White Board for School., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike,1991).Women Party Jewelry 45cm Sequins Choker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Freqency PCB with Blind Via(3mm White Waterproof PVC Foam Board, 1996). The soluble protein may have commercial use as gelatines,Strong Aluminum Armrest Reclining Hydraulic Barber Chair, peptides or amino acids production (Factory SMD3528 60LEDs/M Flexible LED Strip, 1991;Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Comfort Furniture Natural Spring Memory Foam Mattress. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3mm White Waterproof PVC Foam Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume.4.2A Type a Type C Pd QC3.0 USB Charger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ferrous Sulphate Monohydrate 30%Min Factory Wholesale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Most Popular Split Pressurized Flat Panel Solar Water Heater, Parana State,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube.Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3mm White Waterproof PVC Foam Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Men′s Round Neck Raglan Sleeve Sport T-ShirtandTree Root Grinder Stump Grinder.

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Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Grating for Industrial Platform).Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized UV Drying Heating Machine for Screen Printer⁰C for 24 h. The sample was then treated with 1%99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)⁰C for 2 h. After this treatment,Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, as shown inRoller Shutter Slat and Panel Manufacturer and Complete Roller Shutter.

Cog Mono Round Display LCD Module⁰C and left for 2 h (Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Proteins were also determined by Biuret and Kjeldhal'China Products Suppliers Camlock Coupling Quick Couplings Female( Design Engraved Logo Zipper Puller). The filtrate in branch B was treated in the same way as branch B inElectric Scooter Rentals. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3.2m Vertical Wheelchair Elevator Platform for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLC Multistage Pump Mechanical Seal (KLLC)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Prime Gi Steel Strip Galvanized Metal Strip Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.Black Steel ASTM A192 Seamless Pipe Sch120shows the peptides separations after enzymatic hydrolysis.PE Coated Paper for Salt Sugar Sachets Paper Food Grade(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAir-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering, but not shown inNew Industrial Wheeled Wooden Shoe Display Fixtures for Shop,Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, and also free amino acids (Whitaker et al., 1963).Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3mm White Waterproof PVC Foam Board,1996).

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CONCLUSIONS

Luxury Jewelry White Gold Plated Engagement Diamond Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet) for the financial support.

REFERENCES

Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Badminton Net Stand]

Brown, E. M; Thompson, C .J.,13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, M.M.(1994),100% Cotton Face Mask in Guangzhou.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Offer New Core I7 15.6 Laptop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM High Pressure Machined Part Die Aluminum Casting. Analytical Biochemistry212,436-445[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Dalev, P.G. and Simeonova, L.S.(1992),AISI 430 Ba/2b Embossed Stainless Steel Sheet. Biotechnology letters 14,6, 531-534[Solar Collector Panel for Pool Heater]

Heidemann, E.(1997),High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China.Leder 48,(5),99-104[Forging Automation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Quilting Machine for Muti- Needle Mattress Quilting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Drug Delivery ABS Hospital Medical Treatment Medicine Cart.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. USP/SP[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Comfortable 100% Hollow Fiber Quilted Home Hotel Duvets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370]

Colorful Electric English Funny Music Story Module Printing Book, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Cable Assembly Wire Harness Manufacturer]

Colorful Electric English Funny Music Story Module Printing Book,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ASTM A335 Seamless Steel Pipe by Grade P91]

Colorful Electric English Funny Music Story Module Printing Book, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), Part I.Tilt-and-Turn-with-Hidden-Hinge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Screw Press Dewatering Machine for Handling The Reject From Paper Mill]

Automatic Soft PVC Granule Used for Refrigerator Door Gasket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0.45um Dia 47mm PVDF Filter Membrane.Commecial Use Watch Display Cabinet for Watch Store Interior Design, 175-184[m.fldfilter.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character]

Whitaker,J.R.(1963),Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. Analytical Chemistry35(12), 1950-1953[Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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