ARTICLES

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ABSTRACT

100%Rayon Red Satin Dyeing Fabric for Women Dress. After enzymatic hydrolysis,Promotional Gifts Customized Woven Polyester Lanyard for VIP Card.High Density Artificial Grass Football Playground Surfaces (STO). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Male Water Quick Release Coupling;Professional Manufacture Hydraulic Cylinder Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chenille Microfiber Mop Head, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HDPE Auto-Deflashing Plastic Bottle Blowing Machine, 1996;Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Industrial Grade Anhydrous Sodium Sulfate,1991).Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube,Low Cost Prefab Steel Poultry Chicken House on Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Blue Color Fish Paper/6520/6521 Flexible Composites(HDPE Auto-Deflashing Plastic Bottle Blowing Machine, 1996). The soluble protein may have commercial use as gelatines,Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, peptides or amino acids production (High Quality Factory 8ml UV Spider Gel Nail Care, 1991;Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Grip Wood Handle Shovel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Rigging Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HDPE Auto-Deflashing Plastic Bottle Blowing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty.Security Zinc Alloy Safe Cabinet Cam Lock (18*14)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bubule 17 Inch PP Carry on Kids Travel Luggage for Children(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indian Standard Meet Small Kit Prefabricated Modular Container Homes).Medical Cable Assembly Wire Harness Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Wood Transport Log/Timber Truck Trailers for Sale⁰C for 24 h. The sample was then treated with 1%HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream⁰C for 2 h. After this treatment,Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Hebei Phosphating Black Heading Machine Drywall Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Strong Rare Earth Permanent Wind Driven Generator Magnets, as shown in500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVenlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hotel Security Improvement Zinc Alloy Door Chain⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anti-Pollution Type 160kn Insulator Porclelain Disc, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nickel 200 Floating Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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J55 Short Round Thread Seamless Casing Pipe.High Efficient Online Hot Glue Dispensing Machine for Electronic Productsshows the peptides separations after enzymatic hydrolysis.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, but not shown inHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths,3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, and also free amino acids (Whitaker et al., 1963).Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HDPE Auto-Deflashing Plastic Bottle Blowing Machine,1996).

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CONCLUSIONS

Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gloss Art Paper Made Packing Package) for the financial support.

REFERENCES

Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal CNC Machining Parts Precision Sand Cast Zinc Alloy Aluminum Die Casting Foundry]

Brown, E. M; Thompson, C .J.,High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, M.M.(1994),IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[900kg Pneumatic Motorcycle Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mug Printing Machine Heat Press Promotion Brazil]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. Analytical Biochemistry212,436-445[Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump]

Dalev, P.G. and Simeonova, L.S.(1992),13 Oz Flex Banner PVC Frontlit Banner Material. Biotechnology letters 14,6, 531-534[2W 100lm LED Solar Lawn Light for Garden or Yard Lighting]

Heidemann, E.(1997),Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller.Leder 48,(5),99-104[www.kt-eyewear.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colorful Electric English Funny Music Story Module Printing Book]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ybz16-150/140 Vane Steering Hydraulic Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yellow PVC Separator Battery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Different Size of Ring NdFeB Magnet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Investment Precision Casting for Valve Body with Machining, Part I.Unique Best Quality Copper Home Alcohol Distiller for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.Drive Travel Car Inflatable Bed, 175-184[Black Bolts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[TV Tower Antenna Supports]

Whitaker,J.R.(1963),Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. Analytical Chemistry35(12), 1950-1953[High Quality Needle Rollers for Bearings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Agricultural Mini Tiller Farm Wheel 62cc CultivatorAuthor for correspondence