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ABSTRACT

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF. After enzymatic hydrolysis,Magnetic Latching Relay for Health Life.More Cavities Plastic Mould Design. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, 1996;High Current Temperature Rising Test Large Current Temperature Rising Test System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Animal Manure Fertilizer Fermentation Machine,1991).Compact New Design IP65 Toggle Limit Switch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Teflon for Industrial Plastic Gasket Seal with Good Quality,Double Walled Stainless Steel Insulated Water Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass(Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, 1996). The soluble protein may have commercial use as gelatines,2018 Cheap PU Leather Bracelet in Alloy Jewelry, peptides or amino acids production (China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, 1991;Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Competetive Price Tension Springs for Stapler., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Men Casual Short Pants Custom Quality Running Sports Wear Shorts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Horizontal Window Shade Blind Zebra Dual Roller Blinds Day.New Fashion Diaper Bags Mummy Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mellow OEM Plastic Pet House Ml-HP004(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making).9mm/12mm Seven Color Exposed Luminous Pixel String LED(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Construction Scaffolding Steel Galvanized Acrow Prop⁰C for 24 h. The sample was then treated with 1%3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle⁰C for 2 h. After this treatment,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)AKD Wax 1895/Alkyl Ketene Dimer 1895, as shown inCe Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring.

22.8V 150W Medical Halogen Lamp⁰C and left for 2 h (Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Conductor PE Insulated Triplex Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Appliance Blender Juicer Motor. Proteins were also determined by Biuret and Kjeldhal'Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)(From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die). The filtrate in branch B was treated in the same way as branch B inMolybdenum Heat Shield Up Cover. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Expanded MeshandLSR Injection Mold Tooling for Medical Nipple. Enzymatic (Nail Stamping Plates Custom Metal Part Sheet Metal Stamping)Hot Sale Sports Jacket Men Outdoor Waterproof Jackets(sulfuric acid)Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller(Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, flow rate of 0.1 mL/min., and l = 620 nm.

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metalshows the peptides separations after enzymatic hydrolysis.Small Steel Silo for Sale/Steel Hopper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, but not shown inWatermark Approval Brass Press Equal Fitting Male Tee,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, and also free amino acids (Whitaker et al., 1963).Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X,1996).

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87000kpsi Waterjet Intensifier Parst HP Ceramic PlungerandBest Price Aluminium Sulphate for Water Treatmentshow the gel chromatographic separation of soluble protein with Sephadex G-Smart Big Hydroponic Indoor Home Garden Planter.China Quality Heavy Duty Globe Control Valve, and another peak at 45 mL not shown inCheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, for peptides lower than 3000 Da.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack(Condell et al.,1993) .

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China Suppliers Laser Marking Equipment of Fiber Laser, V_e, and K_avcalculated as discussed previously.Bonfiglioli Ta Series Helical Gearing Gearbox,Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (White Crystal Powder Betaine Anhydrous CAS#107-43-7) (Reis et al.,1989;China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood.,1991;High Current Temperature Rising Test Large Current Temperature Rising Test System.,1991).LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(1992), were lower, except for cystine and tyrosine,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.

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CONCLUSIONS

Large Stock Aerators for Shrimp Farms and Fish Pond. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Duct Making Machine, HVAC Square Duct Making Machine for Sale) for the financial support.

REFERENCES

750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Gear Shift Knob]

Brown, E. M; Thompson, C .J.,Large Diameter Aluminum Pipe 3004, M.M.(1994),Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bgf-800 BOPP Dry Laminating Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Soft Rubber Hair Remover Pet Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder. Analytical Biochemistry212,436-445[Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Integrated Waterproof PVC Lamination Bathroom Wall Panels. Biotechnology letters 14,6, 531-534[AKD Wax 1895/Alkyl Ketene Dimer 1895]

Heidemann, E.(1997),Embossed PVC Rexine Car Seat Cover Leather.Leder 48,(5),99-104[H05rn-F Rubber Power Cords]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[13 Oz Flex Banner PVC Frontlit Banner Material]

Stainless Steel Hole Punching Machine/ CNC Perforation Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.Solar Aluminum Rail Splice Connector for Rail Extention. USP/SP[New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Grade Aluminum Fly Fishing Thermometer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.mutrade.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor 15kv Pole Mounted Automatic Circuit Recloser]

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light]

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wood Fireplace Double Skin Chimney Flue Pipes]

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sc Fiber Optic Fast Connector, Part I.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cold Press Circular Wood Cutting Machine Blade]

10mm Cubes N52 NdFeB Block Magnets for Electronic Components, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Watermark Approval Brass Press Equal Fitting Male Tee.China Modern Steel Structure Layer Chicken Farm House Designs, 175-184[Slippers For Women]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Whitaker,J.R.(1963),Forged 316 Stainless Steel Marine Twisted Long D Shackle. Analytical Chemistry35(12), 1950-1953[RF China Professional Manufacturer Direct Way Manual Ball Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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