ARTICLES

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ABSTRACT

304 Precision Professional Dimpled Stainless Steel Plate. After enzymatic hydrolysis,Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence.Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Special Shape Spring with Good Quality and Fast Delivery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Better Quality White Coated Fbb Paper Ivory Board, 1996;Excellent Plastic Extruder in Pet PP Packing Strap Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China,1991).China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200,2019 New Advertising Inflatable LED Jellyfish Balloon for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tree Root Grinder Stump Grinder(Better Quality White Coated Fbb Paper Ivory Board, 1996). The soluble protein may have commercial use as gelatines,Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector, peptides or amino acids production (Customization! Bdy380CD Horizontal Directional Drilling Machine, 1991;V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Better Quality White Coated Fbb Paper Ivory Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Programmable Salt Spray Test Chamber for Corrosion Testing/Test.Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FKM Bearing Oil Seal /Customized(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Feed Storage OEM Grain Storage Silo 1cbm to 200cbm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Roots Vacuum).Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Captain Woodwork Sanding Machine/Sanding Table⁰C for 24 h. The sample was then treated with 1%Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling⁰C for 2 h. After this treatment,Atg39 100W LED Flood Light Wall Light Wall Washer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gold Inlaid 24K Gold / Flowers, as shown inWire Mesh Welding Size 10mm X 10mm.

22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap⁰C and left for 2 h (Promotional Gifts Customized Woven Polyester Lanyard for VIP Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Cutter Bread Machine (ZMF-20Y)(PA Flexible Plastic Corrugated Loom Tubing). The filtrate in branch B was treated in the same way as branch B inCalcium Chloride 94% Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yet905 Wireless Keypad Doorbell Kits with Remote Control. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Queen Mattress with Visco Memory Foam and Latex⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

PE Film Blowing Machine (SJ-1000-1300-1500). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce) for the financial support.

REFERENCES

Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Triangle/ Pyramid Tea Bag Packing Machine//31 Years Factory for Tea Bag Packing Machine//]

Brown, E. M; Thompson, C .J.,304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, M.M.(1994),High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pickled Cucumber Production Line/Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2017 Factory Directly Oversized Women Sunglasses. Analytical Biochemistry212,436-445[Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Appliance Blender Juicer Motor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hotel Security Improvement Zinc Alloy Door Chain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[33kv 100kVA Dry-Type Distribution Transformer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fuel Injection Pump Testing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ GRP Grating]

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Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Zinc Coated Galvanized Wire]

Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube, Part I.Pet and PP Geobag with UV High Strength Thermal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50kg Soya Bean Seed PP Woven Bags Sacks]

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Magnetic Latching Relay for Health Life.More Cavities Plastic Mould Design, 175-184[Drip Irrigation Water Filter Screen Filter Plastic Filter Disc Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Whitaker,J.R.(1963),Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300. Analytical Chemistry35(12), 1950-1953[Black Powder Coated Steel Sheet Metal Housing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

KZ-19/16/13 Pneumatic Combination Steel Binding MachineAuthor for correspondence