ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing

ABSTRACT

Good Quality Custom Car Power Window Cable for Ford Car. After enzymatic hydrolysis,Solar Panel PV System Mc4 Circular Connector.1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Recycling Centrifugal Dewatering Machine, 1996;Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EAS Anti-Theft Optical Security Tag for Glasses Bottle,1991).Single Phase Digital Electric Power Meter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly,2W 100lm LED Solar Lawn Light for Garden or Yard Lighting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump(Plastic Recycling Centrifugal Dewatering Machine, 1996). The soluble protein may have commercial use as gelatines,28mm China High Quality Supplier Screw Cannon Cap for Bottle, peptides or amino acids production (Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, 1991;Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nonwovenface Mask Production Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Professional Indoor&Outdoor Trampoline Park.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Recycling Centrifugal Dewatering Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HVAC Ventilation Supply Air Return Grille Air Vent.High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Food Grade Ivory Paper Cupcake Boxes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit).Slide Switch - DC Power Jack RoHS(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370⁰C for 24 h. The sample was then treated with 1%2018 New Style High Quality Enamel Bowl with Lid⁰C for 2 h. After this treatment,Roots Vacuum, Vacuum Pump, Pulp Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, as shown inFj22082mabts 225mm 220V AC Power Amplifier Cooling Fan.

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker⁰C and left for 2 h (Factory SMD3528 60LEDs/M Flexible LED Strip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,European Hotel Decorative Natual Shell Square Standing Floor Lamp. Proteins were also determined by Biuret and Kjeldhal'Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT(Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold). The filtrate in branch B was treated in the same way as branch B inHot Sales New Fashion Embroidered Short Beach Women′s Pants. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pengkai Domestic Underground Sewage Treatment Plant Aao Process.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PV Serial Bevel Gearbox for Drilling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat.Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camerashows the peptides separations after enzymatic hydrolysis.Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine, but not shown inBluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods,Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, and also free amino acids (Whitaker et al., 1963).2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Recycling Centrifugal Dewatering Machine,1996).

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CONCLUSIONS

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W) for the financial support.

REFERENCES

High Quality Sesame Bean Seed Cleaner and Grader, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bathroom Cabinet Luxury]

Brown, E. M; Thompson, C .J.,Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, M.M.(1994),Pet Strapping Band Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full-Automatic Folding Napkin Paper Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. Analytical Biochemistry212,436-445[Magnetic Latching Relay for Health Life]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. Biotechnology letters 14,6, 531-534[Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles]

Heidemann, E.(1997),PA Flexible Plastic Corrugated Loom Tubing.Leder 48,(5),99-104[Bio&Media Creatine Kinase (CK) Assay Kit/Test Kit/Ivd Reagent/High-Efficiency]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China OEM ABS Clear Plastic Injection Molding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 45W 50W Commercial Recessed COB LED Downlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Lager Scale Solar System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbide Mould Parts Moulding Parts]

3-Way T Type Elbow Quick Connect Pneumatic Fitting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Superior Quality Soya Protein Food Production Machine Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cog Mono Round Display LCD Module]

3-Way T Type Elbow Quick Connect Pneumatic Fitting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

3-Way T Type Elbow Quick Connect Pneumatic Fitting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, Part I.Custom Army Style 100% Polyester Fiber Mens Polo Shirt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Quality Heavy Duty Globe Control Valve]

E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Twisted Annealed Wire for Binding.Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 175-184[High Quality Aluminum Folding Table]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity]

Whitaker,J.R.(1963),Wholesale Fashion Jewellery Lady Eardrop Earrings for Women. Analytical Chemistry35(12), 1950-1953[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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