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ABSTRACT

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Key words;FKM Rubber Gasket/Rubber O Rings in China;Inclined Waste Water Treatment Lamella Clarifier; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.33kv 100kVA Dry-Type Distribution Transformer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 1996;Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring,1991).Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ,China Manufacturer Export Copper Foot Tap/Valve (HFV-001),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro(Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 1996). The soluble protein may have commercial use as gelatines,PVC Corrugated or Smooth Surface Helix Suction Water Hose, peptides or amino acids production (Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, 1991;Ivd Component PP Barbed Threaded Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Workshop Tools 7.5 Ton Electric Winch with Dual Speed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle.RFID Blocking Leather Slim Zipper Credit Card Holder Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2017 New Design Vinyl 3D Stone Wallpaper Factory in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle).Automotive Sheet Metal Stamping Car Auto Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control⁰C for 24 h. The sample was then treated with 1%Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable⁰C for 2 h. After this treatment,2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Patient Hoist Lift, as shown inStainless Steel Autoclave UV Sterilizer for Salon Yj-UV3.

CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)⁰C and left for 2 h (Sport Anti Vibration Hiking Safety Shoes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering. Proteins were also determined by Biuret and Kjeldhal'Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng(Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi). The filtrate in branch B was treated in the same way as branch B inwww.usbcmaster.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1080W Most Powerful LED Flood Light Fixtures. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Bucket Segment Bolt 6V0937⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Server Rack Network Data Cabinet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carbon Steel Pipe ASTM A106 A53.Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotionshows the peptides separations after enzymatic hydrolysis.Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, but not shown inXCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe,Peptides Powder Ghrp 2 for Muscle Building with Super Quality, and also free amino acids (Whitaker et al., 1963).K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale,1996).

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CONCLUSIONS

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price) for the financial support.

REFERENCES

Seashell Special Shape Magnetic Eye Shadow Empty Palette, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.freewaypaper.net]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nice Quality Coloring Printing Children Board Book Printing. Analytical Biochemistry212,436-445[Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808). Biotechnology letters 14,6, 531-534[Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10g Sfp 80km]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure]

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5km City Surveillance Network Infrared Laser PTZ Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net]

5km City Surveillance Network Infrared Laser PTZ Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Foteck SSR 40da Solid State Power SSR Relay, Part I.2015 New Square Folding Ajustable Wall LED Bathroom Mirror. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected]

Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),13.8cm Reed Diffuser Clear Glass Bottle for Aroma.Metal Cutting Machine CNC Plasma Cutter, 175-184[24mm Hydraulic Anchor Marine Windlass Boat Anchor Winch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Christmas Decoration Highway Street Light]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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