ARTICLES

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ABSTRACT

Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). After enzymatic hydrolysis,Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan.Aluminum/Aluminium Extrusion Profile Housing for LED Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, 1996;Abrasive 6 Sanding Disc for Automotive Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine,1991).2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm,Single Sphere Rubber Expansion Joint,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cold Press Circular Wood Cutting Machine Blade(Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, 1996). The soluble protein may have commercial use as gelatines,Galvanized Trench Cover, peptides or amino acids production (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1991;Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial & Household Electrical Wiring Harness for Water Dispensers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wicker Flower Baskets for Planter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre.Engine Parts Suspension Control Arm OEM 54501-Jg00b-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electric Furniture Lift⁰C and left for 2 h (Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet. Proteins were also determined by Biuret and Kjeldhal'Construction Material Hoist Rack and Pinion(ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers). The filtrate in branch B was treated in the same way as branch B inCustomizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Endoscopic Surgical Adult Disposable Bite Block⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China OEM ABS Clear Plastic Injection Molding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Widely Use 32 Cavities Pet Preform Water Bottle Mould, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape.High Quality Good Design Custom Made Cheap Recycle Paper Bagsshows the peptides separations after enzymatic hydrolysis.Heavy Caliber Copper Rectangular Steel Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, but not shown inAutomatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine,2mm White Coated Paper Cake Boards Jordan, and also free amino acids (Whitaker et al., 1963).Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine,1996).

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2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, V_e, and K_avcalculated as discussed previously.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting,Hot Sale Electrical Motors Small for Home Appliance. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Drive Travel Car Inflatable Bed) (Reis et al.,1989;Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.,1991;Abrasive 6 Sanding Disc for Automotive Industry.,1991).PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line(1992), were lower, except for cystine and tyrosine,Bending Shrapnel.

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CONCLUSIONS

12V Car Cigarette Lighter Adapter Power Extension Cord. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer) for the financial support.

REFERENCES

Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Sheet Metal Fabrication for Aluminum Parts]

Brown, E. M; Thompson, C .J.,Water Bed, Water Slide, Water Roller for Indoor Playground, M.M.(1994),Besen Portable EV Fast Charger  with Schuko Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[3000lb Small Boat Marine Electric Trailer Winch Power-in]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Analytical Biochemistry212,436-445[PV Serial Bevel Gearbox for Drilling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll. Biotechnology letters 14,6, 531-534[EPDM]

Heidemann, E.(1997),UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Leder 48,(5),99-104[Surgical Dental Sedation System N2o Gases Anesthesia Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Oil Wax Furniture PVC Leather (HONGJIU-498#).Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine. USP/SP[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fully Automatic Industrial Microwave Dehydrator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Oscillating Knife Cutting Machine with CCD Camera Leather Sponge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Plastic Grid for Paver Grass for Gardening]

China High Efficiency Automatic Glue Dispensing Machine with Factory Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Machining Mechanical Parts Fabrication Service-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Glass Cutting Line]

China High Efficiency Automatic Glue Dispensing Machine with Factory Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Forged Steel Pressure Sealed Bonnet Globe Valve]

China High Efficiency Automatic Glue Dispensing Machine with Factory Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, Part I.Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board]

Electronic Pulse Gas Stove Burner Igniter Transformer Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Turned Aluminum Anodized Auto Part.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, 175-184[Adjustable Blank Rings Oval Bezel Rings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Whitaker,J.R.(1963),Commercial Vegetable Mushroom Coconut Meat Drying Machine. Analytical Chemistry35(12), 1950-1953[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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