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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Extra Heavy Wall Thickness Seamless Tubes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Shrink Sleeves Labels for Packaging Labeling

ABSTRACT

IP65 Canopy LED Light Fixture 150W LED Gas Station Light. After enzymatic hydrolysis,Plastic Mould Dongguan Molding Factory.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004;Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Banded Type Tee, 1996;Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector,1991).Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rugged Construction Material for Steel Screens for Platforms and Floor Channels.,FKM Rubber Gasket/Rubber O Rings in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Inclined Waste Water Treatment Lamella Clarifier(Good Quality Banded Type Tee, 1996). The soluble protein may have commercial use as gelatines,33kv 100kVA Dry-Type Distribution Transformer, peptides or amino acids production (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 1991;Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Banded Type Tee, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacturer Export Copper Foot Tap/Valve (HFV-001).Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Corrugated or Smooth Surface Helix Suction Water Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

New Patient Hoist Lift(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Bucket Segment Bolt 6V0937.Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboardshows the peptides separations after enzymatic hydrolysis.Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, but not shown inShengya Steel Concrete Electric Pole Mould,Outdoor Server Rack Network Data Cabinet, and also free amino acids (Whitaker et al., 1963).Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality Banded Type Tee,1996).

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OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, V_e, and K_avcalculated as discussed previously.Carbon Steel Pipe ASTM A106 A53,Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector) (Reis et al.,1989;Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale.,1991;Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.,1991).Peptides Powder Ghrp 2 for Muscle Building with Super Quality(1992), were lower, except for cystine and tyrosine,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.

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CONCLUSIONS

EPDM, PVC Car or Door Rubber Sealing Strip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Workshop Tools 7.5 Ton Electric Winch with Dual Speed) for the financial support.

REFERENCES

Black Color Tube Eyelet Lift Gas Spring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ie3 Induction Three Phase Fan Motor]

Brown, E. M; Thompson, C .J.,Safety Edge Sensor for Agv Automated Guided Vehicle, M.M.(1994),Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets.The Journal of the American Leather Chemists Association (JALCA)89(7)[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Design Felt Belt Type Cooling Table/Handling Extrusion System. Analytical Biochemistry212,436-445[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

Dalev, P.G. and Simeonova, L.S.(1992),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood. Biotechnology letters 14,6, 531-534[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

Heidemann, E.(1997),Ycom USB Charger and Data Cable with Aluminum for Type C.Leder 48,(5),99-104[m.gsrfid.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine.8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input. USP/SP[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Seashell Special Shape Magnetic Eye Shadow Empty Palette: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Psd Food Grading Biodiesel Centrifugal Separator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Manufacturer Garbage Incinerator]

on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nice Quality Coloring Printing Children Board Book Printing, Part I.Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating.Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 175-184[GM]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Whitaker,J.R.(1963),Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen. Analytical Chemistry35(12), 1950-1953[Customized Factory Supply Stainless Steel T Shape Bolt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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