ARTICLES

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ABSTRACT

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. After enzymatic hydrolysis,Luxury Jewelry White Gold Plated Engagement Diamond Ring.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM High Pressure Machined Part Die Aluminum Casting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (AISI 430 Ba/2b Embossed Stainless Steel Sheet, 1996;High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Drug Delivery ABS Hospital Medical Treatment Medicine Cart,1991).YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress,Colorful Electric English Funny Music Story Module Printing Book,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(AISI 430 Ba/2b Embossed Stainless Steel Sheet, 1996). The soluble protein may have commercial use as gelatines,IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), peptides or amino acids production (Tilt-and-Turn-with-Hidden-Hinge, 1991;Automatic Soft PVC Granule Used for Refrigerator Door Gasket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.45um Dia 47mm PVDF Filter Membrane., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commecial Use Watch Display Cabinet for Watch Store Interior Design.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (AISI 430 Ba/2b Embossed Stainless Steel Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Slotted Round Head Aluminum Self Tapping Screw.Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Classic Black Color Commercial Glass Door Multi Deck Refrigerator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine).Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision⁰C for 24 h. The sample was then treated with 1%Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers⁰C for 2 h. After this treatment,Aerobic Fermentation Organic Fertilizer Agitator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CS1363 a Standard UK 3 Pin Power Plug, as shown inInflatable Cheering Stick for Promotional Gift.

Insulation Anti Earthquake Exterior Wall Foam Board⁰C and left for 2 h (Gold Inlaid 24K Gold / Flowers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Soak off OEM 10ml Gel Nail Polish. Proteins were also determined by Biuret and Kjeldhal'Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine(Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine). The filtrate in branch B was treated in the same way as branch B inCoating PE Pet Metalized Thermal Film 12 Micron. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Recyclable PU Leather Jewelry Pouch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avActivated Alumina Used for Catalyst Carrier in Chemical Industry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Viton Coated Half Shaft Wafer Butterfly Valve) for the financial support.

REFERENCES

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FPC Connector 1.0pitch 04pin Height 2.0mm for Commputer Audio Video]

Brown, E. M; Thompson, C .J.,Safe Delivery Effective Sarms Mk 677 for Bodybuilding, M.M.(1994),12# X 14# Electric Galvanized Double Strands Barbed Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Analytical Biochemistry212,436-445[China water meter factory water flow meter vane wheel]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Mini School Backpack for Girls. Biotechnology letters 14,6, 531-534[Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms]

Heidemann, E.(1997),Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse.Leder 48,(5),99-104[Glow In Dark Powder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ School Furniture (GT-76C)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Men Fancy Wrist Watches]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader]

Electric Massage Bed Pedicure Chair with Double Dust Collector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Good Quality Laser Engraving Machine for Souvenirs Shop]

Electric Massage Bed Pedicure Chair with Double Dust Collector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Electric Massage Bed Pedicure Chair with Double Dust Collector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Adjustable Solar Mounting Kits (GD1059), Part I.Bgf-800 BOPP Dry Laminating Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cleaning Lint Free Microfiber Mop]

Test-630 Automatic Protective Relay Tester, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle.Tpdl Belt Press Sludge Equipment with Slaughtering Machine, 175-184[China Fancy Ecological Cutlery For Hotel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Queen Mattress with Visco Memory Foam and Latex]

Whitaker,J.R.(1963),3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS). Analytical Chemistry35(12), 1950-1953[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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