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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Claus Process Catalyst 4-6 mm. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4 Different Ball Silicone Ice Cube Maker

ABSTRACT

High Quality Washable Durable Hotel Polyester Banquet Table Cloths. After enzymatic hydrolysis,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female.Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aerobic Fermentation Organic Fertilizer Agitator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, 1996;CS1363 a Standard UK 3 Pin Power Plug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Insulation Anti Earthquake Exterior Wall Foam Board,1991).Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Easy Soak off OEM 10ml Gel Nail Polish,Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Recyclable PU Leather Jewelry Pouch(Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, 1996). The soluble protein may have commercial use as gelatines,Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), peptides or amino acids production (Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1991;High Quality 45W 50W Commercial Recessed COB LED Downlight, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.High Speed Auto Corrugated Carton Box Paperboard Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Waterproof BNC Connector for Industry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave).2000LTR Horizontal Foam Tank Fire Fighting Suppression(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Spped Weaving Machine 6⁰C for 24 h. The sample was then treated with 1%Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc⁰C for 2 h. After this treatment,Small Poultry Pig Feed Farm Steel Silo for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, as shown inPolypropylene Fibre for Commercial Slabs.

Made in China 12 Inch Aluminum Flexible Duct Pipe⁰C and left for 2 h ( Lotion Noodle Jam). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. Proteins were also determined by Biuret and Kjeldhal'Safe Delivery Effective Sarms Mk 677 for Bodybuilding(Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly). The filtrate in branch B was treated in the same way as branch B inm.industry-boiler.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12# X 14# Electric Galvanized Double Strands Barbed Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Mini School Backpack for Girls(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Super Potassium Humate Shiny Flakes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4K Giant Whiteboard Interactive Panel for University Whiteboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

White 4*8FT 18mm PVC Foam Board for Cabinets Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solid Steel Hotel Safe (G-42BD classics) for Hotel) for the financial support.

REFERENCES

Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electronic Voting Devices Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, M.M.(1994),Power Plant Circulating Water Pump & Spare Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[Truck Air Dryer cartridge 14bar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. Analytical Biochemistry212,436-445[2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin]

Dalev, P.G. and Simeonova, L.S.(1992),Carbon Steel Equal Tee for Connection of Pipe. Biotechnology letters 14,6, 531-534[XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe]

Heidemann, E.(1997),Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile.Leder 48,(5),99-104[Simple Design Hotel Banquet Meeting Metal Dining Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Horizontal Window Shade Blind Zebra Dual Roller Blinds Day]

Full Viton Coated Half Shaft Wafer Butterfly Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy.Steel Stacking Restaurant Wedding Chair. USP/SP[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heavy Caliber Copper Rectangular Steel Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rutile Anatase Titanium Dioxide for Coating and Paints: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Densen Customized China Transmission Shaft Coupling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Green Agate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Walled Stainless Steel Insulated Water Bottle]

Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Di003 Environmental Protection Against The Stench Floor Drain-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Laser Cutting Machine for Sale for Die Shops From Yitai Die Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks]

Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, Part I.Vacuum Type Single Facer Corrugator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4 Different Ball Silicone Ice Cube Maker]

Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, 175-184[Mobile Phone Case Black Cover for Huawei Y6 2018/ Enjoy 8e/ Honor 7A/ Y6 Prime 2018]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Precision Tooling Grinding Machine Part]

Whitaker,J.R.(1963),125mm Wet Diamond Pad for Polishing Marble. Analytical Chemistry35(12), 1950-1953[Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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