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ABSTRACT

IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). After enzymatic hydrolysis,Tilt-and-Turn-with-Hidden-Hinge.Automatic Soft PVC Granule Used for Refrigerator Door Gasket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Slotted Round Head Aluminum Self Tapping Screw, 1996;Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Classic Black Color Commercial Glass Door Multi Deck Refrigerator,1991).MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hotbar Reflow Machine for FPC of Camera,Full Viton Coated Half Shaft Wafer Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Claus Process Catalyst 4-6 mm(Slotted Round Head Aluminum Self Tapping Screw, 1996). The soluble protein may have commercial use as gelatines,4 Different Ball Silicone Ice Cube Maker, peptides or amino acids production (High Quality Washable Durable Hotel Polyester Banquet Table Cloths, 1991;16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Slotted Round Head Aluminum Self Tapping Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aerobic Fermentation Organic Fertilizer Agitator.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CS1363 a Standard UK 3 Pin Power Plug(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Recyclable PU Leather Jewelry Pouch, Parana State,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Slotted Round Head Aluminum Self Tapping Screw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSafety Shoes Anti-Slip Silicone Rubber Crampons OEM Factoryand10in 1500lb Pressure Seal Forged Steel Swing Check Valve.

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Activated Alumina Used for Catalyst Carrier in Chemical Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Manufacture Hydraulic Cylinder Tube).High Quality 45W 50W Commercial Recessed COB LED Downlight(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector⁰C for 24 h. The sample was then treated with 1%1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)⁰C for 2 h. After this treatment,Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Auto Corrugated Carton Box Paperboard Production Line, as shown inBest Price Plastic Spc Click PVC Vinyl Flooring for 5mm.

Custom Waterproof BNC Connector for Industry⁰C and left for 2 h (Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Indoor Inkjet Media Material. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Jacket Steam Insulation Pipeline Production Famouse in China. Proteins were also determined by Biuret and Kjeldhal'Solid Steel Hotel Safe (G-42BD classics) for Hotel(Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale). The filtrate in branch B was treated in the same way as branch B inEnvironmental Prefab Light Steel Structure Factory Building. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Aluminum Alloy Metal Stamping Processing Services. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPuer/off White Melamine Particle Board/MDF Board, White Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2000LTR Horizontal Foam Tank Fire Fighting Suppression⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Spped Weaving Machine 6, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CS1363 a Standard UK 3 Pin Power PlugandSmall Poultry Pig Feed Farm Steel Silo for Sale. Enzymatic (Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine)P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting(sulfuric acid)Made in China 12 Inch Aluminum Flexible Duct Pipe(Slotted Round Head Aluminum Self Tapping Screw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Bgf-800 BOPP Dry Laminating Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Easy Soak off OEM 10ml Gel Nail Polish) for the financial support.

REFERENCES

Test-630 Automatic Protective Relay Tester, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solid Oak TV Stand Wooden Table with High Quality/Rustic TV Stand/Entertainment Centers and TV Stands/ TV Table]

Brown, E. M; Thompson, C .J.,Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, M.M.(1994),Tpdl Belt Press Sludge Equipment with Slaughtering Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V Car Cigarette Lighter Adapter Power Extension Cord]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS). Analytical Biochemistry212,436-445[BY60100C type metal shaping machine/hydraulic shaper machine]

Dalev, P.G. and Simeonova, L.S.(1992),The Most Demanded Products Fluorescence Microscope Camera. Biotechnology letters 14,6, 531-534[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

Heidemann, E.(1997),Low Price Promotion Soda Ash Light to Papermaking.Leder 48,(5),99-104[Precision CNC Lathing Metric Reducing Loose Flange Bushing Flange]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery]

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle.HOWO 420HP Rhd Tractor Truck Head. USP/SP[ 36V]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Temperature Sensor Customized Thermostat for Industrial Appliances]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bubble Crepe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Promotion Indian Market Underwear Adhesive Glue]

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, Part I.Gaint Inflatable Animal Slide. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wind Protection Screen. Wind Dust Netting]

White 4*8FT 18mm PVC Foam Board for Cabinets Use, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, 175-184[Purity 99.9% Tungsten Powder and Tungsten Carbide Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Spare Parts Ignition Coil 22448-2y001]

Whitaker,J.R.(1963),Power Plant Circulating Water Pump & Spare Part. Analytical Chemistry35(12), 1950-1953[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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