ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Women Summer Swimsuit Chest Zip Bathing Suit. After enzymatic hydrolysis,Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art.Female Header Conn Ptich 1.0mm Connector Btb. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, 1996;Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Warm Paste Packaging Machine,1991).Stainless Steel Resin Trap Strainer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Bar Fridge with Glass Door for Beverage and Beer,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Loader Xt860 Backhoe Loader with Digger(Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, 1996). The soluble protein may have commercial use as gelatines,2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, peptides or amino acids production (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, 1991;Lithium Battery 20ah Pouch 3c Cell 3.7V, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Handweel Three-Way Non-Retention Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Factory Supply Industrial Machine Pasta. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager.500kg Capacity 14m Height Semi Electric Scissor Lift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:for Bahrain Market Hot Cafe Vending Machine F303V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, Parana State,High Zinc Coated Galvanized Wire.2019 Kiet Hydraulic Pipe Bender Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReflective Tape for Vehicle Reflective Markings (tape-02)andFactory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.

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PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Delphi Control Valve 28239295 (622B)).Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED UFO Highbay 250W, 22500 Lumens⁰C for 24 h. The sample was then treated with 1%Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved⁰C for 2 h. After this treatment,Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather, as shown inHigh Speed Auto Corrugated Carton Box Paperboard Production Line.

Cleaning Lint Free Microfiber Mop⁰C and left for 2 h (Solar DC Water Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. Proteins were also determined by Biuret and Kjeldhal'LED Emergency Exit Light Rechargeable 220V(Safety Traffic Rubber Roof Support Feet Products). The filtrate in branch B was treated in the same way as branch B inHydraulic Concrete Placing Boom (PB28A3R). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Firefighting Equipment Aluminum Rescue Tripod with Winch 30m. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cjx2-F (LC1-F) AC Magnetic Contactor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wicker Flower Baskets for Planter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Glass Cutting Line) for the financial support.

REFERENCES

5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Electric Winches 1000kg Lifting]

Brown, E. M; Thompson, C .J.,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, M.M.(1994),Engine Parts Suspension Control Arm OEM 54501-Jg00b.The Journal of the American Leather Chemists Association (JALCA)89(7)[50Hz/60Hz Low Frequency Home Power Inverter 5000W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Besen Portable EV Fast Charger  with Schuko Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat. Analytical Biochemistry212,436-445[Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Sausage Meat Smoker Oven/Smokerhouse Oven]

Cracking Acidification Clay Stabilizer Pale Yellow Liquid, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer.Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife. USP/SP[Various Bridge NdFeB Motor Magnets with High Performance]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Programmable Salt Spray Test Chamber for Corrosion Testing/Test]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Plastic Conduit Gas Water Irrigation LDPE HDPE PE Tube Hose Pipe Extrusion Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

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Customized Thickness EVA High Density Foam EVA Roll, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbide Mould Parts Moulding Parts, Part I.Compression Mens Customized Gym Shorts Sportswear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder.Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 175-184[Manufacturer Custom Print Design Winter Knitted Cotton Waistcoat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Expanded Mesh]

Whitaker,J.R.(1963),P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display. Analytical Chemistry35(12), 1950-1953[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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