ARTICLES

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ABSTRACT

100% PVC Resin PVC Clear Transparent Film for Shower Curtain. After enzymatic hydrolysis,Hotel Security Improvement Zinc Alloy Door Chain.Anti-Pollution Type 160kn Insulator Porclelain Disc. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nickel 200 Floating Ball Valve;3-Layer PE Coating Production Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, 1996;Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wooden Queen/King Size Japan Sofa Bed G1162,1991).J55 Short Round Thread Seamless Casing Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine,3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear(Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, 1996). The soluble protein may have commercial use as gelatines,Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), peptides or amino acids production (Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, 1991;Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nordic Solid Wood Dining Chair/Smile Chair.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Experienced and Professional Plastic Helmet Mold Manufacturer.Private Label OEM Best Cute Waterproof Lip Pluming Gloss-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%TPU TPR Rubber Band Plastic Extruder Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWheel Loader Transmission Gear Box/Customized Casting Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Spare Parts Ignition Coil 22448-2y001⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Good Quality Laser Engraving Machine for Souvenirs Shop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Piggery Farm Pig/Sow Gestation Equipment From China Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet) for the financial support.

REFERENCES

Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Community IC Access Card Induction ID Time Card Smart Card]

Brown, E. M; Thompson, C .J.,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, M.M.(1994),Children Gift Outdoor Wooden Rocking Horse Toy.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Price Promotion Soda Ash Light to Papermaking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Bar Fridge with Glass Door for Beverage and Beer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330. Analytical Biochemistry212,436-445[Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow]

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Heidemann, E.(1997),Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.Leder 48,(5),99-104[Hand Roll Up Piano]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lithium Battery 20ah Pouch 3c Cell 3.7V]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.corediamtool.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Powder Spray Booth for Metal Furniture]

Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13]

Outdoor High Neck Kids Boots Casual Rubber Men Work Boots,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Automatic AAC Concrete Lightweight Block Making Machine]

Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, Part I.2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Different Size of Ring NdFeB Magnet]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330]

Whitaker,J.R.(1963),GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. Analytical Chemistry35(12), 1950-1953[2017 Hot Diped Galvanized Welded Gabion Basket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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