ARTICLES

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ABSTRACT

High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line. After enzymatic hydrolysis,Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring.Nitinol Disposable Guide Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Crane Hook Qtz250 (7032) with 70m Jib Length, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, 1996;Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar,1991).Online High Sensitivity Checkweigher with Pusher Bar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Piggery Farm Pig/Sow Gestation Equipment From China Factory,Lw250*700 Spiral Discharge Decanter Separator for Water Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events(China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, 1996). The soluble protein may have commercial use as gelatines,Children Gift Outdoor Wooden Rocking Horse Toy, peptides or amino acids production (Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330, 1991;TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Disposable Clear Take Away Plastic Salad Bowl Forming Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro).Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP⁰C for 24 h. The sample was then treated with 1%Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge⁰C for 2 h. After this treatment,Carbon Stee Semless Pipe (ASTM A106 Gr B). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, as shown inBeach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.

Hot Selling House Floor Cleaning 360 Spin Mop⁰C and left for 2 h (0.45um Dia 47mm PVDF Filter Membrane). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Advanced Unlocking Tool for Locksmith. Proteins were also determined by Biuret and Kjeldhal'High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts(Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults). The filtrate in branch B was treated in the same way as branch B inSealing Screw/Seal Screw/Screw/Machine Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Design Sleeveless Fitness Wear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC Colorful Battery Operated Small Hand Fan for Outdoor Use. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHsc Heavy Duty 5 Ton Chain Pulley Block(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/48V 200ah UPS Battery Backup for Telecom, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Plastic Grid for Paver Grass for Gardening, V_e, and K_avcalculated as discussed previously.Explosion Proof Rechargeable LED Searchlight,Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disposable Food Packaging Paper Soup Bowl with Lids) (Reis et al.,1989;Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.,1991;Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.,1991).Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair(1992), were lower, except for cystine and tyrosine,Soft Rubber Hair Remover Pet Brush.

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CONCLUSIONS

Forging Metal Building Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Grinding Machining Parts High Precision) for the financial support.

REFERENCES

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shopping Online Printed Customized Fluorescent Packing Plastic Poly Bag]

Brown, E. M; Thompson, C .J.,Safety Traffic Rubber Roof Support Feet Products, M.M.(1994),Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ride on Toys Riding Animals in The Mall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. Analytical Biochemistry212,436-445[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Dalev, P.G. and Simeonova, L.S.(1992),Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter. Biotechnology letters 14,6, 531-534[Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging]

Heidemann, E.(1997),Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture.Leder 48,(5),99-104[Steel Pipe Rate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Single Spindle Micro CNC Lathe Ck0640, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15).Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. USP/SP[Electric Massage Bed Pedicure Chair with Double Dust Collector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rubber Tire Plastic Recycling Shredder with Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[RFID Blocking Leather Slim Zipper Credit Card Holder Wallet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Advanced Technology EPE Plastic Foam Sheet Making Machinery Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheapest Container Living House/Office/Kiosk]

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)]

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Frequency Online UPS W/3W-20K]

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, Part I.Industrial Mens Cotton Reflective Tape Work Pants. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yet905 Wireless Keypad Doorbell Kits with Remote Control]

Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Coil with AA3003 AA3105 H14 H16 H18.High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, 175-184[Industrial Floorings Coating Chromium Oxide Green 1308-38-9]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling]

Whitaker,J.R.(1963),DC Stand Fan Motor PCBA Card 24V. Analytical Chemistry35(12), 1950-1953[Integrated Waterproof PVC Lamination Bathroom Wall Panels]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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