ARTICLES

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ABSTRACT

China Products Suppliers Camlock Coupling Quick Couplings Female. After enzymatic hydrolysis,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.3.2m Vertical Wheelchair Elevator Platform for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LC Multistage Pump Mechanical Seal (KLLC);Wedding Tent Cooling System Outdoor Event Air-Con Units; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, 1996;Prime Gi Steel Strip Galvanized Metal Strip Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fireproof Aluminum Curtain Wall Panel,1991).Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Adjustable Grooming Table(Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, 1996). The soluble protein may have commercial use as gelatines,High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, peptides or amino acids production (PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 1991;Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100% Cotton Face Mask in Guangzhou(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly).OEM High Pressure Machined Part Die Aluminum Casting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%AISI 430 Ba/2b Embossed Stainless Steel Sheet⁰C for 24 h. The sample was then treated with 1%High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China⁰C for 2 h. After this treatment,Drug Delivery ABS Hospital Medical Treatment Medicine Cart. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, as shown inFlat Roof Insulation Board Heat Insulation Board.

Colorful Electric English Funny Music Story Module Printing Book⁰C and left for 2 h (125mm Wet Diamond Pad for Polishing Marble). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). Proteins were also determined by Biuret and Kjeldhal'Tilt-and-Turn-with-Hidden-Hinge(Di003 Environmental Protection Against The Stench Floor Drain). The filtrate in branch B was treated in the same way as branch B in5 Inch Check Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Soft PVC Granule Used for Refrigerator Door Gasket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%0.45um Dia 47mm PVDF Filter Membrane. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommecial Use Watch Display Cabinet for Watch Store Interior Design(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Slotted Round Head Aluminum Self Tapping Screw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Classic Black Color Commercial Glass Door Multi Deck Refrigerator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CS1363 a Standard UK 3 Pin Power Plug, V_e, and K_avcalculated as discussed previously.Insulation Anti Earthquake Exterior Wall Foam Board,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Truck Air Dryer cartridge 14bar) (Reis et al.,1989;PE Coated Paper for Salt Sugar Sachets Paper Food Grade.,1991;Prime Gi Steel Strip Galvanized Metal Strip Price.,1991).Easy Soak off OEM 10ml Gel Nail Polish(1992), were lower, except for cystine and tyrosine,Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.

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CONCLUSIONS

Recyclable PU Leather Jewelry Pouch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic) for the financial support.

REFERENCES

Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Model 75X75mm Continuous LED Linear Light Aluminum Linear Lighting Fixtures]

Brown, E. M; Thompson, C .J.,Activated Alumina Used for Catalyst Carrier in Chemical Industry, M.M.(1994),High Quality 45W 50W Commercial Recessed COB LED Downlight.The Journal of the American Leather Chemists Association (JALCA)89(7)[XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Horizontal Window Shade Blind Zebra Dual Roller Blinds Day]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector. Analytical Biochemistry212,436-445[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

Dalev, P.G. and Simeonova, L.S.(1992),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). Biotechnology letters 14,6, 531-534[Heavy Caliber Copper Rectangular Steel Tube]

Heidemann, E.(1997),Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.Leder 48,(5),99-104[Specialized Wood Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Densen Customized China Transmission Shaft Coupling]

Cog Mono Round Display LCD Module, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.High Speed Auto Corrugated Carton Box Paperboard Production Line. USP/SP[Double Walled Stainless Steel Insulated Water Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Waterproof BNC Connector for Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Threaded Reducing Flange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4 Different Ball Silicone Ice Cube Maker]

Indoor Inkjet Media Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steel Jacket Steam Insulation Pipeline Production Famouse in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Precision Tooling Grinding Machine Part]

Indoor Inkjet Media Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring]

Indoor Inkjet Media Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solid Steel Hotel Safe (G-42BD classics) for Hotel, Part I.Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000]

CNC Aluminum Alloy Metal Stamping Processing Services, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Puer/off White Melamine Particle Board/MDF Board, White Board.Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, 175-184[Bottom Sander Supplier & Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer]

Whitaker,J.R.(1963),2000LTR Horizontal Foam Tank Fire Fighting Suppression. Analytical Chemistry35(12), 1950-1953[Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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