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ABSTRACT

Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. After enzymatic hydrolysis,Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch.Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag;ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Sesame Bean Seed Cleaner and Grader, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1996;Pet Strapping Band Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label,1991).Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PA Flexible Plastic Corrugated Loom Tubing,Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power(Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1996). The soluble protein may have commercial use as gelatines,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, peptides or amino acids production (3-Way T Type Elbow Quick Connect Pneumatic Fitting, 1991;China Superior Quality Soya Protein Food Production Machine Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Army Style 100% Polyester Fiber Mens Polo Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Twisted Annealed Wire for Binding.Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Fashion Jewellery Lady Eardrop Earrings for Women(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, Parana State,120PCS/M Flexible LED Strip Tira De LED Light Strip.China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theConstruction Material Hoist Rack and PinionandChina Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.

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High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbon Steel Spherical Plain Radical Bearings).Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Low Volume Production Aluminum CNC Machining Auto Parts⁰C for 24 h. The sample was then treated with 1%Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple⁰C for 2 h. After this treatment,New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbon Steel Round/Square Pipe Tube Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Green PVC Coated Front Yard Double Wire Mesh Fence Panel, as shown in Carbon Steel 20g Gr. B Steam Cooling Fin Tube.

Cable Length 11 Gun Safety Padlock⁰C and left for 2 h (China Factory Price Hg1 Self Adjustable Pipe Tank Rotator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Canvas School College Backpack/Bookbags for Girls/Students/Women. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. Proteins were also determined by Biuret and Kjeldhal'Women′s Seamless Strapless Bra(Puer/off White Melamine Particle Board/MDF Board). The filtrate in branch B was treated in the same way as branch B inModern Fabric Shade Floor Lamp and Wooden Tripod Fancy Stand Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSilent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AAandFiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Enzymatic (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal)20 Experiences on Plastic Injection Moulding Service(sulfuric acid)Wind Protection Screen. Wind Dust Netting, Wind Dust Net(Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%24K Gold Rolling Paper Shine Papers Cigarette Paper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%24K Gold Rolling Paper Shine Papers Cigarette Paper, flow rate of 0.1 mL/min., and l = 620 nm.

Two - Way Double Row Thrust Tapered Roller Bearings.Pickled Cucumber Production Line/Making Machineshows the peptides separations after enzymatic hydrolysis.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDurable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, but not shown inHigh Quality Factory 8ml UV Spider Gel Nail Care,Ride on Toys Riding Animals in The Mall, and also free amino acids (Whitaker et al., 1963).Finely Processed Pneumatic Operated Globe Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag,1996).

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CONCLUSIONS

Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Automatic AAC Concrete Lightweight Block Making Machine) for the financial support.

REFERENCES

Retractable Multi Core Cable Reel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Licence Plate Frame]

Brown, E. M; Thompson, C .J.,New Xcm 120 Asphalt Mixing Plant for Sale, M.M.(1994),Indian Standard Meet Small Kit Prefabricated Modular Container Homes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Powder Coated Steel Sheet Metal Housing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings. Analytical Biochemistry212,436-445[KZ-19/16/13 Pneumatic Combination Steel Binding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),LED Indoor Light Linear Light Aluminium LED Profile. Biotechnology letters 14,6, 531-534[12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery]

Heidemann, E.(1997),50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price.Leder 48,(5),99-104[1j0919051b Car Auto Spare Parts Auto Parts Fuel Pump Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Blue Silica Gel with Indicating]

Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Graphic 240X128 Dots Monochrome LCD Display Module.Dry & Wet Concrete Surface Metal Grinding Wing. USP/SP[8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Workbench Anti-Static Table ESD Natural Rubber Table Mat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Conductor PE Insulated Triplex Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Elegant Leather Dining Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment]

316L 10 Micron Sintered Stainless Steel Filter Disc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets]

316L 10 Micron Sintered Stainless Steel Filter Disc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB]

316L 10 Micron Sintered Stainless Steel Filter Disc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Abstract Light and Shadow Metal Oil Painting for Deco, Part I.Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Round/Square Pipe Tube Production Line]

Cheapest Container Living House/Office/Kiosk, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Spin Centrifugal Demister Separator or Column Internal.Baby Wet Wipe Machine, 175-184[D8tc High Performance 3-Electrode Motorcycle Spark Plug for Honda]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector]

Whitaker,J.R.(1963),Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. Analytical Chemistry35(12), 1950-1953[Single Spindle Micro CNC Lathe Ck0640]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

FKM Bearing Oil Seal /CustomizedAuthor for correspondence