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ABSTRACT

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. After enzymatic hydrolysis,Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape.Heavy Caliber Copper Rectangular Steel Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxious Hotel Passenger Elevator, Titanium Elevator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yuchai 400kw Diesel Generator Set, 1996;Black Plastic GRP FRP PE Pipe Fitting for Water Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2194230012 for Mercedes Benz Auto Spare Parts Brake Disc,1991).Precision Casting Factory Foundry OEM Ductile Iron Sand Casting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Electrical Motors Small for Home Appliance,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bending Shrapnel(Yuchai 400kw Diesel Generator Set, 1996). The soluble protein may have commercial use as gelatines,12V Car Cigarette Lighter Adapter Power Extension Cord, peptides or amino acids production (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1991;Water Bed, Water Slide, Water Roller for Indoor Playground, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Besen Portable EV Fast Charger  with Schuko Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yuchai 400kw Diesel Generator Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Oil Wax Furniture PVC Leather (HONGJIU-498#)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CNC Turned Aluminum Anodized Auto Part(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Crusher for Animal Waste/ Chicken Manure).Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Commercial Vegetable Mushroom Coconut Meat Drying Machine⁰C for 24 h. The sample was then treated with 1%4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass⁰C for 2 h. After this treatment,Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hydraulic Fold-Bend Machine Hydraulic Bending Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Delphi Control Valve 28239295 (622B), as shown inHigh Efficiency Air Cooled Air Source Water Chiller Heat Pump.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White and Green Board, School Writing Board, School Furniture (GT-76C). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCurrent Precise LED Digital Ammeter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP Polypropylene Two Layer Film Blowing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Sugarcane Pulp Paper Bowl 500ml, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machining Mechanical Parts Fabrication Service) for the financial support.

REFERENCES

High Density Insulation Rock Wool Board for Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Factory High Quality Cheap Acid Alkali Resistant Hose]

Brown, E. M; Thompson, C .J.,Garbage Bag Making Machine Price Trash Bag Sealing Machine, M.M.(1994),12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Patient Hoist Lift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar. Analytical Biochemistry212,436-445[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

Dalev, P.G. and Simeonova, L.S.(1992),Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. Biotechnology letters 14,6, 531-534[Comfort Furniture Natural Spring Memory Foam Mattress]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Crusher for Animal Waste/ Chicken Manure: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Grinding Machining Parts High Precision]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Water Tank Level Sensor GRP Water Tank Stainless Steel Water Tank Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Threaded Locking Circular Connectors M16 680-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality and Efficient Combustion UHP Graphite Electrode]

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, Part I.Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price]

Disposable Sterile Safety Blood Lancet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plush Comfort Spring Mattress Cheap Price for Wholesale.3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, 175-184[Aluminum Honeycomb Composite Panel for Construction Materials]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Whitaker,J.R.(1963),Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. Analytical Chemistry35(12), 1950-1953[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Double Cereal Airtight Food DispenserAuthor for correspondence