ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Sesame Bean Seed Cleaner and Grader. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag

ABSTRACT

Pet Strapping Band Production Line. After enzymatic hydrolysis,UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label.Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PA Flexible Plastic Corrugated Loom Tubing;Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, 1996;3-Way T Type Elbow Quick Connect Pneumatic Fitting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Superior Quality Soya Protein Food Production Machine Line,1991).Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Army Style 100% Polyester Fiber Mens Polo Shirt,E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Twisted Annealed Wire for Binding(Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, 1996). The soluble protein may have commercial use as gelatines,Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, peptides or amino acids production (Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, 1991;High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic AAC Concrete Lightweight Block Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball ValveAlcalase2.5 L DX,High Quality Low Volume Production Aluminum CNC Machining Auto Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Carbon Steel Round/Square Pipe Tube Production Line, Parana State,Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.Green PVC Coated Front Yard Double Wire Mesh Fence Panel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom BackpackandOriginal Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.

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Cable Length 11 Gun Safety Padlock(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Compression Mens Customized Gym Shorts Sportswear).Canvas School College Backpack/Bookbags for Girls/Students/Women(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles⁰C for 24 h. The sample was then treated with 1%Women′s Seamless Strapless Bra⁰C for 2 h. After this treatment,Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100), as shown inBest Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng.

(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station⁰C and left for 2 h (Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes. Proteins were also determined by Biuret and Kjeldhal'Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting(Low Price Promotion Soda Ash Light to Papermaking). The filtrate in branch B was treated in the same way as branch B inLow Price Water Air Cooler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20 Experiences on Plastic Injection Moulding Service(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24K Gold Rolling Paper Shine Papers Cigarette Paper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Two - Way Double Row Thrust Tapered Roller Bearings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Small Bar Fridge with Glass Door for Beverage and BeerandRide on Toys Riding Animals in The Mall. Enzymatic (Finely Processed Pneumatic Operated Globe Valve)Color Coated Corrugated Galvanized Roofing Steel Plate(sulfuric acid)Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting(Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Graphic 240X128 Dots Monochrome LCD Display Module. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple) for the financial support.

REFERENCES

Dry & Wet Concrete Surface Metal Grinding Wing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Capacity Tennis Backpack with Shoes Compartment]

Brown, E. M; Thompson, C .J.,Workbench Anti-Static Table ESD Natural Rubber Table Mat, M.M.(1994),316L 10 Micron Sintered Stainless Steel Filter Disc.The Journal of the American Leather Chemists Association (JALCA)89(7)[2017 Hot Diped Galvanized Welded Gabion Basket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Analytical Biochemistry212,436-445[Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices]

Dalev, P.G. and Simeonova, L.S.(1992),Abstract Light and Shadow Metal Oil Painting for Deco. Biotechnology letters 14,6, 531-534[Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer]

Heidemann, E.(1997),Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).Leder 48,(5),99-104[Codice macchina stampante]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Hebei Phosphating Black Heading Machine Drywall Screw]

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheapest Container Living House/Office/Kiosk.Spin Centrifugal Demister Separator or Column Internal. USP/SP[Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Baby Wet Wipe Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Magnifying Wall-Mounted Swivel Bathroom Folding Make up Vanity Mirror]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier]

Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen]

Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear]

Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:360 Degree Reciprocating Car Wash Machine, Part I.Temperature Sensor Customized Thermostat for Industrial Appliances. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor]

Custom Die Aluminum Castings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Meat Pie Making Machine with Different Mould Shape.Gear Operated Flanged High Performance Butterfly Valve, 175-184[www.hhomedecor.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wheel Loader Transmission Gear Box/Customized Casting Parts]

Whitaker,J.R.(1963),China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Analytical Chemistry35(12), 1950-1953[DC Stand Fan Motor PCBA Card 24V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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