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ABSTRACT

12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. After enzymatic hydrolysis,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Single Block Vertical Wire Drawing Machine for Screw and Bolt Making;China Professional Shipping Agent to Turkey; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Crusher for Animal Waste/ Chicken Manure, 1996;Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Threaded Locking Circular Connectors M16 680,1991).Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card,Disposable Sterile Safety Blood Lancet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plush Comfort Spring Mattress Cheap Price for Wholesale(Crusher for Animal Waste/ Chicken Manure, 1996). The soluble protein may have commercial use as gelatines,3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, peptides or amino acids production (Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, 1991;Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof LED Flash Shoe Light Crystal Epoxy Resin. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Crusher for Animal Waste/ Chicken Manure, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Shopping Paper Bag for Clothing Package.Qingdao Us Type Load Binder Lever Chain Tensioner-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Elbow SS304 SS316L(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Upper Limbs Machine/Spare Parts for Fitness Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aerobic Fermentation Organic Fertilizer Agitator).Cvkr Silent Check Valve Dn350(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Shatterproof Gold Rim Wine Glasses⁰C for 24 h. The sample was then treated with 1%10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting⁰C for 2 h. After this treatment,High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil, as shown inHigh Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lamella Clarifier for Wastewater Treatment Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTube Cutting Machine CNC Fiber Laser Metal Pipe Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter MachineandE6000 Handheld Three Phase Power Quality and Energy Analyzer. Enzymatic (Qg Jg Fg Series Air Dryer Equipment Factory)Motor Bearing 6014 2RS Deep Groove Ball Bearing(sulfuric acid)PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line(Crusher for Animal Waste/ Chicken Manure, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pet Short Fiber Non Woven Geotextile for Landfill Filtration, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pet Short Fiber Non Woven Geotextile for Landfill Filtration, flow rate of 0.1 mL/min., and l = 620 nm.

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Jaguar Jb-390 Automatic PCB Loader and Unloader, V_e, and K_avcalculated as discussed previously.Scew Weighing Scale Electronic Counting Scale,China Factory Organic Ethyl Ascorbic Acid. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ferrous Sulphate Monohydrate 30%Min Factory Wholesale) (Reis et al.,1989;Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.,1991;Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs.,1991).Aluno Aluminum Veneer Price Aluminium Curtain Wall Details(1992), were lower, except for cystine and tyrosine,Factory Price Sodium Gluconate 98% for Concrete Admixture.

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CONCLUSIONS

Pneumatic Cut off Ball Valve with Flange Thread Weld End. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack) for the financial support.

REFERENCES

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Globe Valve]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. Analytical Biochemistry212,436-445[Bending Shrapnel]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Grating for Industrial Platform. Biotechnology letters 14,6, 531-534[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Heidemann, E.(1997),50Hz/60Hz Low Frequency Home Power Inverter 5000W.Leder 48,(5),99-104[Xanthan Gum Food Additive Viscosity 1200]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5 Gallon Pure Water Bottle Non-Spill Cap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heading Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lint Free Nail Gel Polish Remover Nonwoven Wipes, Part I.Shs250X500 Galvanized Square Rectangular Steel Tube Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe.Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, 175-184[Factory Manufacturer 100 Ml Cosmetic Lotion Perfume Plastic Pet Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

Whitaker,J.R.(1963),From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die. Analytical Chemistry35(12), 1950-1953[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized Steel Grating for MetallurgyAuthor for correspondence