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ABSTRACT

Hotel Security Improvement Zinc Alloy Door Chain. After enzymatic hydrolysis,Anti-Pollution Type 160kn Insulator Porclelain Disc.Nickel 200 Floating Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3-Layer PE Coating Production Line;Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, 1996;Wooden Queen/King Size Japan Sofa Bed G1162., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (J55 Short Round Thread Seamless Casing Pipe,1991).2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)(Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, 1996). The soluble protein may have commercial use as gelatines,Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, peptides or amino acids production (Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1991;IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nordic Solid Wood Dining Chair/Smile Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Experienced and Professional Plastic Helmet Mold Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Private Label OEM Best Cute Waterproof Lip Pluming Gloss.Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chubby 163cm Fat Big Ass Female Real Sex Doll for Man(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (T Slot Aluminum Extrusion Ob4040b Aluminium Profile).Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10mm AISI304 Carbon Steel Balls Motor Bearing for Slide⁰C for 24 h. The sample was then treated with 1%Different Size of Ring NdFeB Magnet⁰C for 2 h. After this treatment,Stainless Steel Investment Precision Casting for Valve Body with Machining. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Unique Best Quality Copper Home Alcohol Distiller for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, as shown inPower Plant Circulating Water Pump & Spare Part.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wheel Loader Transmission Gear Box/Customized Casting Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Good Quality Laser Engraving Machine for Souvenirs Shop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game MachineandReverse Flow Configuration Axial Fan. Enzymatic (Handcraft Wood Jewelry Storage Box with Lock and Key)TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit(sulfuric acid)Single Spindle Micro CNC Lathe Ck0640(Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tent Windows Use China Made PVC Transparent Clear Mesh Tarp) for the financial support.

REFERENCES

Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dc Electric Motor For Treadmill Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Children Gift Outdoor Wooden Rocking Horse Toy, M.M.(1994),Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Promotion Indian Market Underwear Adhesive Glue]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. Analytical Biochemistry212,436-445[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Dalev, P.G. and Simeonova, L.S.(1992),Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Biotechnology letters 14,6, 531-534[Wind Protection Screen. Wind Dust Netting]

Heidemann, E.(1997),ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L.Leder 48,(5),99-104[1000W Ipg Laser Source Sheet and Pipe/Tube Metal Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Spare Parts Ignition Coil 22448-2y001]

Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration.XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. USP/SP[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor High Neck Kids Boots Casual Rubber Men Work Boots: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Nonwoven Foldable Duckbill Dust Face Mask]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC Wood Grain Decorative Sheet]

Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat]

Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, Part I.CNC Grinding Machining Parts High Precision. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump]

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag.GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, 175-184[2.4 LCD Baby Monitor Wireless Video Baby Camera with Night Vision Intercom Temperature Display Lullaby GB101]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike]

Whitaker,J.R.(1963),Disposable Clear Take Away Plastic Salad Bowl Forming Machine. Analytical Chemistry35(12), 1950-1953[Document Storage Durable Metal Wire Mesh Decking for Pallet Rack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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