ARTICLES

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ABSTRACT

Nice Quality Coloring Printing Children Board Book Printing. After enzymatic hydrolysis,Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808).Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating;Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5km City Surveillance Network Infrared Laser PTZ Camera, 1996;300 M Portable Water Well Mining Exploration Core Drilling Rig., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Foteck SSR 40da Solid State Power SSR Relay,1991).2015 New Square Folding Ajustable Wall LED Bathroom Mirror. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle,13.8cm Reed Diffuser Clear Glass Bottle for Aroma,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metal Cutting Machine CNC Plasma Cutter(5km City Surveillance Network Infrared Laser PTZ Camera, 1996). The soluble protein may have commercial use as gelatines,Medical Suction Connecting Tube with Yankauer Suction Handle, peptides or amino acids production (Surface Mounted Wall Light for Home Decoration Waterproof, 1991;Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Self-Propelled Wheel Farm Using 3 Rows Corn Harvester., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Right Angle Worm Gearbox for Food and Beverage and Packaging Machines.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacturer High Strength Carbon Steel Heavy Hex Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5km City Surveillance Network Infrared Laser PTZ Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine.Stainless Steel Seamless Coil Tube ASTM A249/A269-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler, Parana State,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.Food Grade Beverage Clear PVC Tubing Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5km City Surveillance Network Infrared Laser PTZ Camera, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Checkered Plate 304 316L 410 Floor MaterialandNarrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.

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Double Coil Control Solenoid Valve of Butterfly Valve⁰C and left for 2 h (Medical Cable Assembly Wire Harness Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Frequency Online UPS W/3W-20K. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solar Collector Panel for Pool Heater. Proteins were also determined by Biuret and Kjeldhal'ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers(ASTM A335 Seamless Steel Pipe by Grade P91). The filtrate in branch B was treated in the same way as branch B inImee Printing Custom Wet Tissue Make up Remover Wipe Wet Wipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Sheet Metal Case Parts for Industrial Computer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Premium quality for Potassium Humate powder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Water Pipe Insulation Steam) for the financial support.

REFERENCES

Customization! Bdy380CD Horizontal Directional Drilling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Simple Cosmetic Train Bag Case with Handle]

Brown, E. M; Thompson, C .J.,V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, M.M.(1994),Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Educational Equipment Whiteboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Graphite Ring Good Lubrication]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment. Analytical Biochemistry212,436-445[ Agrochemicals]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification. Biotechnology letters 14,6, 531-534[Small Brown Glass Bottles]

Heidemann, E.(1997),Programmable Salt Spray Test Chamber for Corrosion Testing/Test.Leder 48,(5),99-104[70 Tons Mining Truck with Brick Crane Qy70K-I]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins]

Seashell Special Shape Magnetic Eye Shadow Empty Palette, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%.FKM Bearing Oil Seal /Customized. USP/SP[High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Marking Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve]

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Coill Nail Making Machine/Coil Nail Welding Machine]

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10mm Tempered Bathroom Glass Safety Glass for Building]

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Max Twintier Rb441t Using Wire Coils Tw1061t, Part I.Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[American NEMA Straight Blade Plug 30A 125V/250V Power Plug]

Manufacturer Supply Durable Grey Color Non-Slip EVA Mat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Feed Storage OEM Grain Storage Silo 1cbm to 200cbm.Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 175-184[Plastic foamed board machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector]

Whitaker,J.R.(1963),Captain Woodwork Sanding Machine/Sanding Table. Analytical Chemistry35(12), 1950-1953[Advanced Unlocking Tool for Locksmith]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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