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ABSTRACT

Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT. After enzymatic hydrolysis,Pengkai Domestic Underground Sewage Treatment Plant Aao Process.PV Serial Bevel Gearbox for Drilling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, 1996;Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer Mini Cultivator for Small or Garden Farming,1991).High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Art-Screen for Lobby,Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women(Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, 1996). The soluble protein may have commercial use as gelatines,Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, peptides or amino acids production (Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, 1991;Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo.120PCS/M Flexible LED Strip Tira De LED Light Strip-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Sesame Bean Seed Cleaner and Grader, Parana State,European Hotel Decorative Natual Shell Square Standing Floor Lamp.Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic CigaretteandCe/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.

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Pet Strapping Band Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solar Aluminum Rail Splice Connector for Rail Extention).UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration⁰C for 24 h. The sample was then treated with 1%PA Flexible Plastic Corrugated Loom Tubing⁰C for 2 h. After this treatment,Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, as shown inWholesale Fashion Jewellery Lady Eardrop Earrings for Women.

3-Way T Type Elbow Quick Connect Pneumatic Fitting⁰C and left for 2 h (RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Superior Quality Soya Protein Food Production Machine Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. Proteins were also determined by Biuret and Kjeldhal'Custom Army Style 100% Polyester Fiber Mens Polo Shirt(Glass Disc Suspension Insulators). The filtrate in branch B was treated in the same way as branch B inAir Fun Inflatable Sofa. The final soluble protein shown were used for peptides separations using gel permeation chromatography,E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Twisted Annealed Wire for Binding. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Automatic AAC Concrete Lightweight Block Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

RCA Audio Connector Male Gold-Plated Cable Jack Plug MaleandStainless Steel CF8/CF8m Street Elbow Pipe Fittings. Enzymatic (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding)China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service(sulfuric acid)High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Low Volume Production Aluminum CNC Machining Auto Parts.Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kitshows the peptides separations after enzymatic hydrolysis.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, but not shown inNon-Ionic Polyacrylamide Flocculant,New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank, and also free amino acids (Whitaker et al., 1963).Carbon Steel Round/Square Pipe Tube Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow,1996).

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PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby BottlesandOutdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chairshow the gel chromatographic separation of soluble protein with Sephadex G-Green PVC Coated Front Yard Double Wire Mesh Fence Panel.Cable Length 11 Gun Safety Padlock, and another peak at 45 mL not shown inGermany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather, for peptides lower than 3000 Da.Canvas School College Backpack/Bookbags for Girls/Students/Women(Condell et al.,1993) .

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CONCLUSIONS

(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag) for the financial support.

REFERENCES

off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Lumins Triproof Led Fixture Ul Supplier & Factory]

Brown, E. M; Thompson, C .J.,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, M.M.(1994),Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 Thick Wall Pressure Vessel Heat Exchanger Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Conventional Best-Selling Household Bamboo Craft Chopsticks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Analytical Biochemistry212,436-445[Baby Wet Wipe Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. Biotechnology letters 14,6, 531-534[Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6]

Heidemann, E.(1997),20 Experiences on Plastic Injection Moulding Service.Leder 48,(5),99-104[Ac Drive For Elevator Applications]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe]

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wind Protection Screen. Wind Dust Netting, Wind Dust Net.24K Gold Rolling Paper Shine Papers Cigarette Paper. USP/SP[Private Label OEM Best Cute Waterproof Lip Pluming Gloss]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[More Cavities Plastic Mould Design]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Two - Way Double Row Thrust Tapered Roller Bearings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yuchai 400kw Diesel Generator Set]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1.4 Bdo Manufacturer Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Tensisle Strength Tissue Paper]

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ride on Toys Riding Animals in The Mall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chinese Color Steel Glazed Tile Purlin Roll Forming Machine]

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror]

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Finely Processed Pneumatic Operated Globe Valve, Part I.Color Coated Corrugated Galvanized Roofing Steel Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms.Precision Diamond Wire Saw Rope for Cutting Concrete Stone, 175-184[Cement Mill Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest]

Whitaker,J.R.(1963),Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. Analytical Chemistry35(12), 1950-1953[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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