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ABSTRACT

Aluminum Sheet Metal Case Parts for Industrial Computer. After enzymatic hydrolysis,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set.High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Premium quality for Potassium Humate powder, 1996;Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Design Solid Wood Double Adult Bed Bedroom Furniture,1991).Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns,304 Precision Professional Dimpled Stainless Steel Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence(Premium quality for Potassium Humate powder, 1996). The soluble protein may have commercial use as gelatines,Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, peptides or amino acids production (Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 1991;Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Special Shape Spring with Good Quality and Fast Delivery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Better Quality White Coated Fbb Paper Ivory Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Excellent Plastic Extruder in Pet PP Packing Strap Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Premium quality for Potassium Humate powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China.China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness).Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Programmable Salt Spray Test Chamber for Corrosion Testing/Test⁰C for 24 h. The sample was then treated with 1%Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%⁰C for 2 h. After this treatment,FKM Bearing Oil Seal /Customized. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, as shown inSuper Potassium Humate Shiny Flakes.

Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce⁰C and left for 2 h (Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Max Twintier Rb441t Using Wire Coils Tw1061t. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm. Proteins were also determined by Biuret and Kjeldhal'Manufacturer Supply Durable Grey Color Non-Slip EVA Mat(4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black). The filtrate in branch B was treated in the same way as branch B inPerforated Stainless Steel Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Feed Storage OEM Grain Storage Silo 1cbm to 200cbm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCaptain Woodwork Sanding Machine/Sanding Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Atg39 100W LED Flood Light Wall Light Wall Washer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gold Inlaid 24K Gold / Flowers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

HVAC Ventilation Supply Air Return Grille Air Ventand22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap. Enzymatic (Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes)Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board(sulfuric acid)Hydraulic Cutter Bread Machine (ZMF-20Y)(Premium quality for Potassium Humate powder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, flow rate of 0.1 mL/min., and l = 620 nm.

Yet905 Wireless Keypad Doorbell Kits with Remote Control.Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335cshows the peptides separations after enzymatic hydrolysis.Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, but not shown inShaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate,Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, and also free amino acids (Whitaker et al., 1963).China Factory Queen Mattress with Visco Memory Foam and Latex. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Premium quality for Potassium Humate powder,1996).

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Ceiling Mounted IV Stand Stainlesss Steel I V Pole, V_e, and K_avcalculated as discussed previously.Pipe Connection Types of Pressing Scaffolding Universal Clamp,Ml33-2mm Eddy Current Linear Position/Displacement Sensor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box) (Reis et al.,1989;Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling.,1991;Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle.,1991).Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat(1992), were lower, except for cystine and tyrosine,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain.

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CONCLUSIONS

Wholesale Long Lasting Custom Private Label Glitter Lip Gloss. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector) for the financial support.

REFERENCES

ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.madcmotor.com]

Brown, E. M; Thompson, C .J.,10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, M.M.(1994),19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Dyed Colored Sand, Natural Coloured Sand. Analytical Biochemistry212,436-445[Reflective Tape for Vehicle Reflective Markings (tape-02)]

Dalev, P.G. and Simeonova, L.S.(1992),China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps. Biotechnology letters 14,6, 531-534[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

Heidemann, E.(1997),Various Bridge NdFeB Motor Magnets with High Performance.Leder 48,(5),99-104[5000L-10000L 3-Layers 4-Layers 5-Layers Large Plastic Blow Molding/Moulding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Auto Corrugated Carton Box Paperboard Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar DC Water Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5-Lead ECG leadwires for philips digitrak plus 24 hour holter 3100A]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Safety Traffic Rubber Roof Support Feet Products]

Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PE Film Blowing Machine (SJ-1000-1300-1500)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, Part I.304 304L 904L 309S 310S Stainless Steel Design Checkered Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reverse Flow Configuration Axial Fan]

High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2017 Factory Directly Oversized Women Sunglasses.High Quanlity PTFE Thread Seal Tape, 175-184[Compost Turner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

Whitaker,J.R.(1963),Fiberglass Grating, FRP Grating, GRP Grating. Analytical Chemistry35(12), 1950-1953[Good Quality Banded Type Tee]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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