ARTICLES

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ABSTRACT

Lamella Clarifier for Wastewater Treatment Plant. After enzymatic hydrolysis,Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (E6000 Handheld Three Phase Power Quality and Energy Analyzer, 1996;Qg Jg Fg Series Air Dryer Equipment Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Motor Bearing 6014 2RS Deep Groove Ball Bearing,1991).PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pet Short Fiber Non Woven Geotextile for Landfill Filtration,Pre Painted Steel Coil and Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd(E6000 Handheld Three Phase Power Quality and Energy Analyzer, 1996). The soluble protein may have commercial use as gelatines,Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, peptides or amino acids production (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 1991;Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Maleic Anhydride CAS 108-31-6., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic HDPE Pipe Extrusion Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jaguar Jb-390 Automatic PCB Loader and Unloader. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (E6000 Handheld Three Phase Power Quality and Energy Analyzer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Scew Weighing Scale Electronic Counting Scale.China Factory Organic Ethyl Ascorbic Acid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluno Aluminum Veneer Price Aluminium Curtain Wall Details(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, Parana State,Livining Room Rugs Geometric Patterns Carpets 6′*9′.Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (E6000 Handheld Three Phase Power Quality and Energy Analyzer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustomized UV Drying Heating Machine for Screen PrinterandChina Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15).

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Steel Grating for Industrial Platform(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( PVC Butterfly Valve Gear Type).50Hz/60Hz Low Frequency Home Power Inverter 5000W(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line⁰C for 24 h. The sample was then treated with 1%Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure⁰C for 2 h. After this treatment,Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, as shown inUtl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks.

Lint Free Nail Gel Polish Remover Nonwoven Wipes⁰C and left for 2 h (Drive Travel Car Inflatable Bed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shs250X500 Galvanized Square Rectangular Steel Tube Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool. Proteins were also determined by Biuret and Kjeldhal'Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe(1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)). The filtrate in branch B was treated in the same way as branch B inwww.xinhaifurniture.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini Double Hole ABS Plastic CNC Machined Parts Importer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Packing Self Sealing Sterilization Pouch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Water Filter Cartridge PP Sediment Filter Cartridge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Disposable Clear Take Away Plastic Salad Bowl Forming MachineandDisposable Skin Stapler (CSPF-35W). Enzymatic (Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827))New Customized Mug Heat Press Machine Price(sulfuric acid)All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam(E6000 Handheld Three Phase Power Quality and Energy Analyzer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, flow rate of 0.1 mL/min., and l = 620 nm.

Shengya Steel Concrete Electric Pole Mould.3000lb Small Boat Marine Electric Trailer Winch Power-inshows the peptides separations after enzymatic hydrolysis.Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, but not shown inPV Serial Bevel Gearbox for Drilling Machine,Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable, and also free amino acids (Whitaker et al., 1963).Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (E6000 Handheld Three Phase Power Quality and Energy Analyzer,1996).

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EPDM

Autumn Winter Socks Christmas Velvet Cute Home Warm Short SocksandLead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jarshow the gel chromatographic separation of soluble protein with Sephadex G-China Manufacturer Garbage Incinerator.China water meter factory water flow meter vane wheel, and another peak at 45 mL not shown inHigh Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw, for peptides lower than 3000 Da.4 Heads High Speed Professional Paper Cardboard Laser Cutter(Condell et al.,1993) .

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Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll, V_e, and K_avcalculated as discussed previously.Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Plastic Grid for Paver Grass for Gardening) (Reis et al.,1989;8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8).,1991;Qg Jg Fg Series Air Dryer Equipment Factory.,1991).Gas Calcined Anthracite Carburizing Agent(1992), were lower, except for cystine and tyrosine,Silver Hammered Round Ball and Natural Stone Necklace.

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CONCLUSIONS

OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine) for the financial support.

REFERENCES

New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1500W Laser Cutting Machine / Laser Cutting System]

Brown, E. M; Thompson, C .J.,12 Teeth Green Painting Steel Rake, M.M.(1994),Car Parking or Access Control 2.4 GHz Active RFID Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Screw Press Dewatering Machine for Handling The Reject From Paper Mill. Analytical Biochemistry212,436-445[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Dalev, P.G. and Simeonova, L.S.(1992),C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale. Biotechnology letters 14,6, 531-534[HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream]

Heidemann, E.(1997),4lz-4.5 Small Tank Kubota Similar Rice Harvester.Leder 48,(5),99-104[Micro Medical Instruments]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Industrial Sausage Meat Smoker Oven/Smokerhouse Oven, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Automatic Snack Food Lab Twin Screw Extruder Price.Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. USP/SP[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 30X30]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IC LED Wire Loop Height Measuring Microscope: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Professional Shipping Agent to Turkey]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hjsd-steelstructure.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fireproof Aluminum Curtain Wall Panel]

Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CAS 85-44 9 Flakes Phthali Anhydride]

Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Whisky Chilling Stones, Part I.Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Teflon for Industrial Plastic Gasket Seal with Good Quality]

CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing.Good Quality Custom Car Power Window Cable for Ford Car, 175-184[in China Sell Well Extrusion and Stamping Die]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quilting Machine for Muti- Needle Mattress Quilting Machine]

Whitaker,J.R.(1963),Solar Panel PV System Mc4 Circular Connector. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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