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ABSTRACT

Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . After enzymatic hydrolysis,China Manufacturer Export Copper Foot Tap/Valve (HFV-001).Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC Corrugated or Smooth Surface Helix Suction Water Hose;Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ivd Component PP Barbed Threaded Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Workshop Tools 7.5 Ton Electric Winch with Dual Speed, 1996;Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim,1991).500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,RFID Blocking Leather Slim Zipper Credit Card Holder Wallet,2017 New Design Vinyl 3D Stone Wallpaper Factory in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Small Paper Gift Box with Magnet Closure for E-Cigarette(Workshop Tools 7.5 Ton Electric Winch with Dual Speed, 1996). The soluble protein may have commercial use as gelatines,Agricultural Mini Tiller Farm Wheel 62cc Cultivator, peptides or amino acids production (0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, 1991;Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficient Online Hot Glue Dispensing Machine for Electronic Products., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Workshop Tools 7.5 Ton Electric Winch with Dual Speed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automotive Sheet Metal Stamping Car Auto Parts.Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker).0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1080W Most Powerful LED Flood Light Fixtures⁰C for 24 h. The sample was then treated with 1%OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery⁰C for 2 h. After this treatment,Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Bucket Segment Bolt 6V0937's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, as shown inHot Sale Sports Jacket Men Outdoor Waterproof Jackets.

Outdoor Server Rack Network Data Cabinet⁰C and left for 2 h (Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House. Proteins were also determined by Biuret and Kjeldhal'High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable(Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid). The filtrate in branch B was treated in the same way as branch B in3 Rpm Gear Motor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Steel Pipe ASTM A106 A53(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Peptides Powder Ghrp 2 for Muscle Building with Super Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EPDM, PVC Car or Door Rubber Sealing Strip, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

5 Gallon Pure Water Bottle Non-Spill CapandBlack Color Tube Eyelet Lift Gas Spring. Enzymatic (Safety Edge Sensor for Agv Automated Guided Vehicle)Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets(sulfuric acid)Best Design Felt Belt Type Cooling Table/Handling Extrusion System(Workshop Tools 7.5 Ton Electric Winch with Dual Speed, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood, flow rate of 0.1 mL/min., and l = 620 nm.

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Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808), V_e, and K_avcalculated as discussed previously.Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine,Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2) (Reis et al.,1989;0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price.,1991;Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment.,1991).Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley(1992), were lower, except for cystine and tyrosine,Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen.

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CONCLUSIONS

5km City Surveillance Network Infrared Laser PTZ Camera. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Patient Hoist Lift) for the financial support.

REFERENCES

300 M Portable Water Well Mining Exploration Core Drilling Rig, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.ironwiremeshfactory.com]

Brown, E. M; Thompson, C .J.,Foteck SSR 40da Solid State Power SSR Relay, M.M.(1994),2015 New Square Folding Ajustable Wall LED Bathroom Mirror.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bathroom Makeup LED Mirror Glass with Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Canvas School College Backpack/Bookbags for Girls/Students/Women]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. Analytical Biochemistry212,436-445[Good Quality Custom Car Power Window Cable for Ford Car]

Dalev, P.G. and Simeonova, L.S.(1992),13.8cm Reed Diffuser Clear Glass Bottle for Aroma. Biotechnology letters 14,6, 531-534[5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment]

Heidemann, E.(1997),Metal Cutting Machine CNC Plasma Cutter.Leder 48,(5),99-104[2 Wheels Foldable Adult Surfing Kugoo Kick Scooter Electric Scooter (ES1201)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Dyed Colored Sand]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automotive OEM Copper Wire Harness and Connectors for Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spo2 Sensor Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Low Volume Production Aluminum CNC Machining Auto Parts]

Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Right Angle Worm Gearbox for Food and Beverage and Packaging Machines-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

Self-Propelled Wheel Farm Using 3 Rows Corn Harvester,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer High Strength Carbon Steel Heavy Hex Nut, Part I.Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy]

Stainless Steel Seamless Coil Tube ASTM A249/A269, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT).Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock, 175-184[Large Bottle Jar Quinko Blow Molding Making Machine Pet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet]

Whitaker,J.R.(1963),Hardware Rigging Forged Red Weldless Pear Shaped Link. Analytical Chemistry35(12), 1950-1953[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC CentreAuthor for correspondence