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ABSTRACT

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor. After enzymatic hydrolysis,Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks.Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Warm Paste Packaging Machine;Stainless Steel Resin Trap Strainer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Bar Fridge with Glass Door for Beverage and Beer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, 1996;Cheap Loader Xt860 Backhoe Loader with Digger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle,1991).Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lithium Battery 20ah Pouch 3c Cell 3.7V,Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Handweel Three-Way Non-Retention Ball Valve(Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, 1996). The soluble protein may have commercial use as gelatines,New Design Factory Supply Industrial Machine Pasta, peptides or amino acids production (Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager, 1991;500kg Capacity 14m Height Semi Electric Scissor Lift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;for Bahrain Market Hot Cafe Vending Machine F303V., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Glass Cutting Line.Small & Tiny CNC Precision Machining Brass Parts Metal Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof LED Flash Shoe Light Crystal Epoxy Resin).Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cleaning Lint Free Microfiber Mop⁰C for 24 h. The sample was then treated with 1%L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm⁰C for 2 h. After this treatment,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Emergency Exit Light Rechargeable 220V's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, as shown inPop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses.

Firefighting Equipment Aluminum Rescue Tripod with Winch 30m⁰C and left for 2 h (3mm Indoor Wall Cladding Decoration Aluminum Composite Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer. Proteins were also determined by Biuret and Kjeldhal'Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter(2mm White Coated Paper Cake Boards Jordan). The filtrate in branch B was treated in the same way as branch B inMilling Metal Parts/ High Volume Production/ CNC Machining Stainess Steel Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cjx2-F (LC1-F) AC Magnetic Contactor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHandmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum/Aluminium Extrusion Profile Housing for LED Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Mode Sc/APC Simplex Fiber Optic Adapter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.Plastic Mould Dongguan Molding Factoryshows the peptides separations after enzymatic hydrolysis.Single Sphere Rubber Expansion Joint(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, but not shown in7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care,Cold Press Circular Wood Cutting Machine Blade, and also free amino acids (Whitaker et al., 1963).Galvanized Trench Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player,1996).

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Small & Tiny CNC Precision Machining Brass Parts Metal PartandLED Indoor Light Linear Light Aluminium LED Profileshow the gel chromatographic separation of soluble protein with Sephadex G-Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, and another peak at 45 mL not shown inPE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow, for peptides lower than 3000 Da.Commercial & Household Electrical Wiring Harness for Water Dispensers(Condell et al.,1993) .

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Wicker Flower Baskets for Planter, V_e, and K_avcalculated as discussed previously.5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D) (Reis et al.,1989;Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager.,1991;Cheap Loader Xt860 Backhoe Loader with Digger.,1991).Engine Parts Suspension Control Arm OEM 54501-Jg00b(1992), were lower, except for cystine and tyrosine,Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat.

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CONCLUSIONS

Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine) for the financial support.

REFERENCES

Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hight Quality Spark Plug for Ngk Bpr6es Toyota/Mazda/Nissan]

Brown, E. M; Thompson, C .J.,Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, M.M.(1994),Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife.The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fully Automatic Digital Pid Temperature Controller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. Analytical Biochemistry212,436-445[Competetive Price Tension Springs for Stapler]

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Heidemann, E.(1997),Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide.Leder 48,(5),99-104[Wolfram Sheet/Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vibrating Condom Ring and Cock Ring Supplier]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot-Press Small Continuous Diamond Saw Blade Cutting Disc]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Drinking Mineral Water Filling Line/Water Bottling Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[General Purpose High Performance Vector Control Frequency Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bianco Carrara Marble]

99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts]

99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, Part I.Electric Furniture Lift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electro Galvanized Low Carbon Straight Cut Wire]

Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet.Construction Material Hoist Rack and Pinion, 175-184[Medical Orthopaedic Disposable Trocar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture]

Whitaker,J.R.(1963),3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. Analytical Chemistry35(12), 1950-1953[Wholesale Professional Indoor&Outdoor Trampoline Park]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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