ARTICLES

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ABSTRACT

Glass Water Jet Cutting Machine (Skwj-4018A). After enzymatic hydrolysis,U. S. Type Clevis Grab Hook.Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Cotton Baby/Kid Hat (DH-BF332), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 1996;OEM Round Stainless Steel Fruit Plate Tray., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Supply Industry Grade Sodium Gluconate,1991).Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube,Mini High Temperature Clothing Labels Screen Printing Machine Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.(Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 1996). The soluble protein may have commercial use as gelatines,Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, peptides or amino acids production (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1991;Conventional Best-Selling Household Bamboo Craft Chopsticks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Marked Medical Syringe Pump (WP08N).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet.CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electro Galvanized Low Carbon Straight Cut Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes, Parana State,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module.Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the33kv 100kVA Dry-Type Distribution Transformerand GRP Grating.

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Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Zinc Coated Galvanized Wire).5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter⁰C for 24 h. The sample was then treated with 1%Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm⁰C for 2 h. After this treatment,White Pattern Acrylic Sheet for Decoration Produced by Senpu. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, as shown in50kg Soya Bean Seed PP Woven Bags Sacks.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolid Carbide Single Flute End Mills Oblique Angle Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Capacity Automatic Cup Filling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

KZ-19/16/13 Pneumatic Combination Steel Binding MachineandSeasoned Cast Iron South Africa Three Legged Potjie Pot. Enzymatic (Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255)Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine(sulfuric acid)2019 New Release Doypack Sachet Powder Packing Machine Price(Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Amplifier Tube Glass Vacuum Tube (833C), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Amplifier Tube Glass Vacuum Tube (833C), flow rate of 0.1 mL/min., and l = 620 nm.

Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools.12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Batteryshows the peptides separations after enzymatic hydrolysis.New Style Custom Logo Makeup Multi Colored Eyeshadow Palette(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlue Silica Gel with Indicating, but not shown in8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8),Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, and also free amino acids (Whitaker et al., 1963).20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line,1996).

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PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, V_e, and K_avcalculated as discussed previously.Hot Promotion Indian Market Underwear Adhesive Glue,700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Carbon Steel Round/Square Pipe Tube Production Line) (Reis et al.,1989;Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.,1991;OEM Round Stainless Steel Fruit Plate Tray.,1991).Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files(1992), were lower, except for cystine and tyrosine,New Industrial Wheeled Wooden Shoe Display Fixtures for Shop.

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CONCLUSIONS

Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stx825 Dual 15 Powerful PA Speaker, Speaker Box) for the financial support.

REFERENCES

Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sintered Stainless Steel Micro Bubble Diffuser]

Brown, E. M; Thompson, C .J.,Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light, M.M.(1994),High Quality Aluminum Carpet Topping Stage Platform.The Journal of the American Leather Chemists Association (JALCA)89(7)[FKM Bearing Oil Seal /Customized]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-BY60100C type metal shaping machine/hydraulic shaper machine. Analytical Biochemistry212,436-445[Vacuum Type Single Facer Corrugator]

Dalev, P.G. and Simeonova, L.S.(1992),Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. Biotechnology letters 14,6, 531-534[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

Heidemann, E.(1997),China Spplier 70W Solar Street Light with Camera Monitoring System.Leder 48,(5),99-104[Agricultural Sprinkler Irrigation Machinery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Heavy-Duty Tape Dispenser Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Explosion Proof Rechargeable LED Searchlight]

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, Part I.Masturbators Adult Sex Toys Japanese Love Doll. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Whitaker,J.R.(1963),12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike. Analytical Chemistry35(12), 1950-1953[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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