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ABSTRACT

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. After enzymatic hydrolysis,Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP.Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbon Stee Semless Pipe (ASTM A106 Gr B);OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling House Floor Cleaning 360 Spin Mop, 1996;Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Advanced Unlocking Tool for Locksmith,1991).High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Design Sleeveless Fitness Wear,DC Colorful Battery Operated Small Hand Fan for Outdoor Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hsc Heavy Duty 5 Ton Chain Pulley Block(Hot Selling House Floor Cleaning 360 Spin Mop, 1996). The soluble protein may have commercial use as gelatines,Outdoor Christmas Decoration Highway Street Light, peptides or amino acids production (Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, 1991;China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;48V 200ah UPS Battery Backup for Telecom., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling House Floor Cleaning 360 Spin Mop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment.Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8L Mini Fridge Freezer for Home and Car Fridge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, Parana State,Disposable Clear Take Away Plastic Salad Bowl Forming Machine.Mining Machinery Parts Diamond Saw Blade Segments for Marble, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Selling House Floor Cleaning 360 Spin Mop, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Factory Offer New Core I7 15.6 Laptopand12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable.

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Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solar Collector Panel for Pool Heater).China Plastic Grid for Paver Grass for Gardening(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Explosion Proof Rechargeable LED Searchlight⁰C for 24 h. The sample was then treated with 1%Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1⁰C for 2 h. After this treatment,Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Soft Rubber Hair Remover Pet Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Forging Metal Building Parts, as shown inStainless Steel Checkered Plate 304 316L 410 Floor Material.

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound⁰C and left for 2 h (Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Safety Traffic Rubber Roof Support Feet Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. Proteins were also determined by Biuret and Kjeldhal'Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(Top Furniture Cheap Iron Stacking Banquet Hall Chairs). The filtrate in branch B was treated in the same way as branch B inMulti Bag Filter Water Treatment System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rubber Tire Plastic Recycling Shredder with Factory Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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DC Stand Fan Motor PCBA Card 24V.Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370shows the peptides separations after enzymatic hydrolysis.Economic Brwon Kraft Slip Sheet Tray and Pallet Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Cable Assembly Wire Harness Manufacturer, but not shown inASTM A335 Seamless Steel Pipe by Grade P91,Stainless Steel 304 Wire Rope Fork Terminal, and also free amino acids (Whitaker et al., 1963).Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling House Floor Cleaning 360 Spin Mop,1996).

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CONCLUSIONS

Fashion Water Drop Earring Charming 925 Silver Earrings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle) for the financial support.

REFERENCES

Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3D Printing Best Quality Digital Printing Polyester Fabric Velvet]

Brown, E. M; Thompson, C .J.,Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks, M.M.(1994),Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nitinol Disposable Guide Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds. Analytical Biochemistry212,436-445[Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. Biotechnology letters 14,6, 531-534[Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag]

Heidemann, E.(1997),Blue Silica Gel with Indicating.Leder 48,(5),99-104[1m Vertical Small Disabled Home Wheelchair Elevator Lift]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Educational Equipment Whiteboard]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Agrochemicals]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Brown Glass Bottles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Industrial Steel I Beam Bending Welding Structure]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins]

Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Precision Green Sand Casting Ball Valve Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB]

Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer]

Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, Part I.Class 300 1/2 Stainless Steel Forging Type Check Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve]

Whitaker,J.R.(1963),Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale. Analytical Chemistry35(12), 1950-1953[Coill Nail Making Machine/Coil Nail Welding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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