ARTICLES

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ABSTRACT

Small Portable Scooters Folding Electric Scooters for Adults. After enzymatic hydrolysis,Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle;Most Popular Split Pressurized Flat Panel Solar Water Heater; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1996;Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized UV Drying Heating Machine for Screen Printer,1991).99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947,RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1996). The soluble protein may have commercial use as gelatines,Cog Mono Round Display LCD Module, peptides or amino acids production (4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, 1991;460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Products Suppliers Camlock Coupling Quick Couplings Female., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3.2m Vertical Wheelchair Elevator Platform for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LC Multistage Pump Mechanical Seal (KLLC).Wedding Tent Cooling System Outdoor Event Air-Con Units-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, Parana State,Ferrous Sulphate Monohydrate 30%Min Factory Wholesale.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335candFast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs.

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Adjustable Grooming Table(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Output Vulcanizing Press Machine).High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PE Coated Paper for Salt Sugar Sachets Paper Food Grade⁰C for 24 h. The sample was then treated with 1%Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood⁰C for 2 h. After this treatment,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, as shown in3-Layer PE Coating Production Line.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury Jewelry White Gold Plated Engagement Diamond Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100% Cotton Face Mask in Guangzhou, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM High Pressure Machined Part Die Aluminum Casting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Super Bass Music TVandAISI 430 Ba/2b Embossed Stainless Steel Sheet. Enzymatic (High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China)Drug Delivery ABS Hospital Medical Treatment Medicine Cart(sulfuric acid)YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, flow rate of 0.1 mL/min., and l = 620 nm.

Colorful Electric English Funny Music Story Module Printing Book.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Boxshows the peptides separations after enzymatic hydrolysis.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTwisted Annealed Wire for Binding, but not shown inSy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse,IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), and also free amino acids (Whitaker et al., 1963).Tilt-and-Turn-with-Hidden-Hinge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine,1996).

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Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, V_e, and K_avcalculated as discussed previously.Slotted Round Head Aluminum Self Tapping Screw,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Delphi Control Valve 28239295 (622B)) (Reis et al.,1989;4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.,1991;Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag.,1991).Classic Black Color Commercial Glass Door Multi Deck Refrigerator(1992), were lower, except for cystine and tyrosine,MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker.

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CONCLUSIONS

Hotbar Reflow Machine for FPC of Camera. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fireproof Aluminum Curtain Wall Panel) for the financial support.

REFERENCES

Full Viton Coated Half Shaft Wafer Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Price 3 Extruders Co-Extrusion of PE Film Blowing]

Brown, E. M; Thompson, C .J.,Claus Process Catalyst 4-6 mm, M.M.(1994),4 Different Ball Silicone Ice Cube Maker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Safety Traffic Rubber Roof Support Feet Products]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Washable Durable Hotel Polyester Banquet Table Cloths. Analytical Biochemistry212,436-445[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

Dalev, P.G. and Simeonova, L.S.(1992),16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Biotechnology letters 14,6, 531-534[Reverse Flow Configuration Axial Fan]

Heidemann, E.(1997),Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe.Leder 48,(5),99-104[Trasmital Kyb Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. USP/SP[Good Quality Banded Type Tee]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Natural OEM Private Label Customized Skin Care Face Serum]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aerobic Fermentation Organic Fertilizer Agitator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Plastic Handle Stainless Steel Kitchen Gadget]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[IN-G702 Single Channel Cosmetics Micro Infusion Hospital Use Syringe Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply]

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CS1363 a Standard UK 3 Pin Power Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Shatterproof Gold Rim Wine Glasses]

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer Supply Durable Grey Color Non-Slip EVA Mat]

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Insulation Anti Earthquake Exterior Wall Foam Board, Part I.Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female]

Easy Soak off OEM 10ml Gel Nail Polish, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.Recyclable PU Leather Jewelry Pouch, 175-184[8inch Galvanized Water Well Johnson Type Screens]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single-Screw Plastic Extruders Machine]

Whitaker,J.R.(1963),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). Analytical Chemistry35(12), 1950-1953[50Hz/60Hz Low Frequency Home Power Inverter 5000W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Besen Portable EV Fast Charger  with Schuko PlugAuthor for correspondence