ARTICLES

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m.yasentag.comEdivaldo E.Garcia^I; Cláudia C.M.Hot Sale Electrical Motors Small for Home Appliance^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Bending Shrapnel

ABSTRACT

12V Car Cigarette Lighter Adapter Power Extension Cord. After enzymatic hydrolysis,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals.Water Bed, Water Slide, Water Roller for Indoor Playground. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996;Oil Wax Furniture PVC Leather (HONGJIU-498#)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine,1991).M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China High Efficiency Automatic Glue Dispensing Machine with Factory Price,CNC Machining Mechanical Parts Fabrication Service,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch(UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996). The soluble protein may have commercial use as gelatines,Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, peptides or amino acids production (Electronic Pulse Gas Stove Burner Igniter Transformer Parts, 1991;CNC Turned Aluminum Anodized Auto Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial Vegetable Mushroom Coconut Meat Drying Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Headband Big Headphone EVA Bag Tool Case Specialized Storage Box.Hydraulic Fold-Bend Machine Hydraulic Bending Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Delphi Control Valve 28239295 (622B)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Current Precise LED Digital Ammeter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IP65 Canopy LED Light Fixture 150W LED Gas Station Light).Automatic Drinking Mineral Water Filling Line/Water Bottling Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men⁰C for 24 h. The sample was then treated with 1%PP Polypropylene Two Layer Film Blowing Machine⁰C for 2 h. After this treatment,Disposable Sugarcane Pulp Paper Bowl 500ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flat Roof Insulation Board Heat Insulation Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, as shown inFiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.

Custom Female Threaded Tee T Shape Pipe Fittings⁰C and left for 2 h (High Density Insulation Rock Wool Board for Wall). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. Proteins were also determined by Biuret and Kjeldhal'Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker(Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports). The filtrate in branch B was treated in the same way as branch B inICU Used Electric Five Function Medical Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural OEM Private Label Customized Skin Care Face Serum⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Screw Press Dewatering Machine for Cassava Residue, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Density Insulation Rock Wool Board for Wall.Food Grade Beverage Clear PVC Tubing Hoseshows the peptides separations after enzymatic hydrolysis.Garbage Bag Making Machine Price Trash Bag Sealing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, but not shown inKd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker,12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable, and also free amino acids (Whitaker et al., 1963).Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,1996).

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Home Design Solid Wood Double Adult Bed Bedroom FurnitureandFire Protection Red Painted Ductile Iron Grooved Pipe Fittingshow the gel chromatographic separation of soluble protein with Sephadex G-Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window.Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, and another peak at 45 mL not shown inHot Sale Loading Truck with Cummins Engine, for peptides lower than 3000 Da.China Professional Shipping Agent to Turkey(Condell et al.,1993) .

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White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, V_e, and K_avcalculated as discussed previously.Crusher for Animal Waste/ Chicken Manure,Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation) (Reis et al.,1989;Electronic Pulse Gas Stove Burner Igniter Transformer Parts.,1991;Oil Wax Furniture PVC Leather (HONGJIU-498#).,1991).Threaded Locking Circular Connectors M16 680(1992), were lower, except for cystine and tyrosine,Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector.

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CONCLUSIONS

Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bathroom Makeup LED Mirror Glass with Light) for the financial support.

REFERENCES

Disposable Sterile Safety Blood Lancet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[pro-lighting.cocn]

Brown, E. M; Thompson, C .J.,Plush Comfort Spring Mattress Cheap Price for Wholesale, M.M.(1994),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss.The Journal of the American Leather Chemists Association (JALCA)89(7)[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. Analytical Biochemistry212,436-445[Customized Hardware Aluminum Alloy Die Casting]

Dalev, P.G. and Simeonova, L.S.(1992),Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Biotechnology letters 14,6, 531-534[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

Heidemann, E.(1997),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.Leder 48,(5),99-104[Smart Hotel Bathroom Mirror LED Broadway Vanity Illuminated Bathroom]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Shopping Paper Bag for Clothing Package: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Powder Stick Bag Film Packing Sealing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food]

Qingdao Us Type Load Binder Lever Chain Tensioner, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Elbow SS304 SS316L-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Automatic Snack Food Lab Twin Screw Extruder Price]

Qingdao Us Type Load Binder Lever Chain Tensioner,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass]

Qingdao Us Type Load Binder Lever Chain Tensioner, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, Part I.Outdoor 15kv Pole Mounted Automatic Circuit Recloser. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input]

Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),14 Heads Double Door Multihead Weigher.Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine, 175-184[Best Selling Cheap Price Custom Logo Blank Metal Printing Nickel Plating Name Button Tin Pin Badges for Staffs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers]

Whitaker,J.R.(1963),White Marble, Carrara White, Bianco Carrara Marble for Sale. Analytical Chemistry35(12), 1950-1953[Precision Casting Factory Foundry OEM Ductile Iron Sand Casting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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