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ABSTRACT

Hydraulic Cutter Bread Machine (ZMF-20Y). After enzymatic hydrolysis,9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board.Yet905 Wireless Keypad Doorbell Kits with Remote Control. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Factory Queen Mattress with Visco Memory Foam and Latex, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, 1996;Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance,1991).Ceiling Mounted IV Stand Stainlesss Steel I V Pole. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pipe Connection Types of Pressing Scaffolding Universal Clamp,Ml33-2mm Eddy Current Linear Position/Displacement Sensor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat(Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, peptides or amino acids production (Wholesale Long Lasting Custom Private Label Glitter Lip Gloss, 1991;ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Dyed Colored Sand, Natural Coloured Sand. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps.Various Bridge NdFeB Motor Magnets with High Performance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Melamine Powder for Industrial Uses(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Magnetic Latching Relay for Health Life. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMore Cavities Plastic Mould Design(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Air Duct Making Machine, HVAC Square Duct Making Machine for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch) for the financial support.

REFERENCES

China Brass Precision CNC Machining Spare Auto Parts with Competitive Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Price 2 1/2 X 4 Inch Carbon Steel Pipe Galvanized Rectangular Tube/2′′ Galvanized Pipe Used for PVC Window and Door]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t. Analytical Biochemistry212,436-445[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Export Copper Foot Tap/Valve (HFV-001)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Meatball Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Blince High Torque OMR Hydro Motor for Injection Moulding Machine]

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AKD Wax 1895/Alkyl Ketene Dimer 1895,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nice Quality Coloring Printing Children Board Book Printing]

AKD Wax 1895/Alkyl Ketene Dimer 1895, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Conductor PE Insulated Triplex Cable, Part I.Home Appliance Blender Juicer Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling]

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 175-184[Calcined Petroleum Coke with Low Sulfur]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mini Double Hole ABS Plastic CNC Machined Parts Importer]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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