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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Forging Metal Building Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound

ABSTRACT

Safety Traffic Rubber Roof Support Feet Products. After enzymatic hydrolysis,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter;Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1996;Rubber Tire Plastic Recycling Shredder with Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft,1991).Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data,Industrial Mens Cotton Reflective Tape Work Pants,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye(Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1996). The soluble protein may have commercial use as gelatines,Aluminum Coil with AA3003 AA3105 H14 H16 H18, peptides or amino acids production (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, 1991;DC Stand Fan Motor PCBA Card 24V, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Economic Brwon Kraft Slip Sheet Tray and Pallet Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel 304 Wire Rope Fork Terminal.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet.Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAS 85-44 9 Flakes Phthali Anhydride(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Non-Ionic Polyacrylamide Flocculant, Parana State,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.Fashion Water Drop Earring Charming 925 Silver Earrings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAws A5.1 E6013 Welding Electrode in Carbon Steel StructureandRugged Construction Material for Steel Screens for Platforms and Floor Channels..

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Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment).Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger⁰C for 24 h. The sample was then treated with 1%I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds⁰C for 2 h. After this treatment,Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Blue Silica Gel with Indicating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system, as shown in40W Integrated Solar Street Lighting LED for Park.

Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock⁰C and left for 2 h (FKM Rubber Gasket/Rubber O Rings in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board. Proteins were also determined by Biuret and Kjeldhal'High Precision Green Sand Casting Ball Valve Parts(Safety Edge Sensor for Agv Automated Guided Vehicle). The filtrate in branch B was treated in the same way as branch B ina-topmachine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Class 300 1/2 Stainless Steel Forging Type Check Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCAD Cam Dental Machine 3D Dental Model Printer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Power Extension Cord AC Cable Plug for Office Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5 Liter Packed Medical Ultrasound Gel with Ce and ISO, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Tube Cutting Machine CNC Fiber Laser Metal Pipe MachineandNew Vmc Machine Auto Parts Processing CNC Vertical Machining Centre. Enzymatic (Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character)Etched Stainless Steel Titanium Sheet(sulfuric acid)Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric(Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Good Design Custom Made Cheap Recycle Paper Bags) for the financial support.

REFERENCES

Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Power Window Switch Fit For Suzuki]

Brown, E. M; Thompson, C .J.,50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation, M.M.(1994),5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[DC Colorful Battery Operated Small Hand Fan for Outdoor Use]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Supply Industry Grade Sodium Gluconate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-600mm Warehouse No Power Roof Fan. Analytical Biochemistry212,436-445[Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar]

Dalev, P.G. and Simeonova, L.S.(1992),Small Brown Glass Bottles. Biotechnology letters 14,6, 531-534[Lw250*700 Spiral Discharge Decanter Separator for Water Treatment]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Mens Cotton Reflective Tape Work Pants]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Best Price Spc Indoor Floor Vinyl Plank Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness]

Stainless Braided PTFE Hose R14 Standard Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Super Potassium Humate Shiny Flakes]

Stainless Braided PTFE Hose R14 Standard Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Stainless Braided PTFE Hose R14 Standard Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black Steel ASTM A192 Seamless Pipe Sch120, Part I.Raw Material Adhesive NBR Latex of Latex Long Gloves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw.Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress, 175-184[22mm Metal Pin Terminal Indicator Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HVAC Ventilation Supply Air Return Grille Air Vent]

Whitaker,J.R.(1963),1200X1000X810 Strong Collapsible Plastic Containers for Storage. Analytical Chemistry35(12), 1950-1953[Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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